Department of Medical Biology and Genetics, Istanbul Bilim University, Istanbul, Turkey.
Leuk Lymphoma. 2011 Jan;52(1):79-84. doi: 10.3109/10428194.2010.531410. Epub 2010 Dec 6.
The Rai and Binet staging systems, which are used as standard methods for evaluating the prognosis of chronic lymphocytic leukemia (CLL), have some restrictions in identifying patients with early-stage CLL who will progress rapidly. To solve this defect, other prognostic parameters have become important in recent years. Intracellular up-regulation of the AID gene in the leukemic lymphocytes of patients with CLL may be an important parameter for predicting the progression of CLL. In this study, AID mRNA expression levels were evaluated in 50 patients with CLL and 50 healthy controls. AID mRNA expression was significantly higher in patients than in controls. We then evaluated AID mRNA levels according to the stages of CLL. Regarding AID mRNA levels, patients with Rai stages 0, I, and II were compared with patients with stages III and IV, whereas patients with Binet stage A were compared with patients with Binet stages B and C. In patients with higher-risk Rai stages III and IV and Binet stages B and C, activation-induced cytidine deaminase (AID) mRNA levels were also significantly higher. Additionally, we found that the mRNA levels of patients with AID in CLL were eight-fold higher than those in control patients, suggesting that AID overexpression promotes chromosomal abnormalities and is associated with CLL progression and survival. For this reason, and because of the simplicity of quantitative real-time PCR analysis, AID might be a useful clinical parameter after its importance is confirmed in larger and multivariate studies.
Rai 和 Binet 分期系统被用作评估慢性淋巴细胞白血病 (CLL) 预后的标准方法，但在识别快速进展的早期 CLL 患者方面存在一些限制。为了解决这个缺陷，近年来其他预后参数变得很重要。CLL 患者白血病淋巴细胞中 AID 基因的细胞内上调可能是预测 CLL 进展的一个重要参数。在这项研究中，我们评估了 50 例 CLL 患者和 50 例健康对照者的 AID mRNA 表达水平。与对照组相比，患者的 AID mRNA 表达明显升高。然后，我们根据 CLL 的分期评估了 AID mRNA 水平。关于 AID mRNA 水平，将 Rai 分期 0、I 和 II 期的患者与 III 和 IV 期的患者进行了比较，而将 Binet 分期 A 的患者与 Binet 分期 B 和 C 的患者进行了比较。在 Rai 分期 III 和 IV 期以及 Binet 分期 B 和 C 期的高危患者中，激活诱导的胞苷脱氨酶 (AID) mRNA 水平也明显升高。此外，我们发现 CLL 患者的 AID mRNA 水平是对照患者的八倍，表明 AID 过表达促进染色体异常，并与 CLL 进展和生存有关。因此，由于定量实时 PCR 分析的简单性，在更大规模和多变量研究中确认其重要性后，AID 可能成为一种有用的临床参数。
