Department of Medical Biology and Genetics, Istanbul Bilim University, Buyukdere Cad No:120 Esentepe, 34394 Istanbul, Turkey.
Med Oncol. 2012 Jun;29(2):1068-72. doi: 10.1007/s12032-011-9821-3. Epub 2011 Jan 25.
Rai and Binet staging systems that have been used as a standard method for evaluating the prognosis of chronic lymphocytic leukemia (CLL) have some restrictions in distinguishing the early stage CLL patients that will progress rapidly. To solve this shortcoming, prognostic parameters other than staging have become important in the recent years. Intracellular upregulation of Fc mu receptor (FCMR, FAIM3/TOSO) gene in the leukemic lymphocytes of the patients with CLL may be an important parameter in predicting the progression of the disease. In this study, FCMR mRNA expression levels were evaluated in 50 CLL patients and in 50 healthy controls. FCMR mRNA expression was found to be significantly higher in CLL patients than in healthy controls. We, then, evaluated FCMR mRNA levels according to the stages of CLL. Rai stage 0, I, II cases were compared with stage III and IV, and Binet A was compared with Binet B and C according to FCMR mRNA levels. In cases with higher risks, Rai stage III, IV and Binet stage B and C, FCMR mRNA levels were also significantly higher. In addition, overexpression of the FCMR seems to be promoting the chromosomal abnormalities. As a result, we found that the mRNA levels of FCMR in the CLL patients are 23-fold higher than that of the control group and this may suggest that it can be associated with the disease progression and survival. For this reason and because of the simplicity of analyzing with Q-PCR, it can be a useful clinical parameter, after its importance has been shown in larger and multi-variate studies.
Rai 和 Binet 分期系统已被用作评估慢性淋巴细胞白血病 (CLL) 预后的标准方法，但在区分将快速进展的早期 CLL 患者方面存在一些限制。为了解决这一缺点，近年来，除分期外的预后参数在 CLL 患者中变得重要。CLL 患者白血病淋巴细胞中 Fc 受体 μ 链（FCMR，FAIM3/TOSO）基因的细胞内上调可能是预测疾病进展的重要参数。在这项研究中，评估了 50 例 CLL 患者和 50 例健康对照者的 FCMR mRNA 表达水平。发现 CLL 患者的 FCMR mRNA 表达明显高于健康对照组。然后，我们根据 CLL 的分期评估了 FCMR mRNA 水平。根据 FCMR mRNA 水平，将 Rai 分期 0、I、II 期与 III 和 IV 期进行比较，将 Binet A 与 Binet B 和 C 进行比较。在风险较高的情况下，Rai 分期 III、IV 和 Binet 分期 B 和 C，FCMR mRNA 水平也明显较高。此外，FCMR 的过度表达似乎促进了染色体异常。结果表明，CLL 患者的 FCMR mRNA 水平比对照组高 23 倍，这可能提示它与疾病进展和生存有关。因此，由于通过 Q-PCR 分析很简单，在更大规模和多变量研究中证明其重要性后，它可能成为一种有用的临床参数。
