Department of Facial Plastic Surgery, Apollo Hospitals, Hyderabad, India.
Dermatol Surg. 2010 Dec;36 Suppl 4:2106-10. doi: 10.1111/j.1524-4725.2010.01702.x.
Botulinum toxin A is considered to be an extremely fragile molecule, with recommended usage of some reconstituted botulinum toxin A formulations within 4 hours of reconstitution. Extreme caution is recommended to avoid agitation of the vial.
To determine whether the effect of reconstituted botulinum toxin A is maintained even when it is agitated vigorously.
Experimental, comparative, animal study.
Sixty-four white Swiss Webster mice, aged 2 to 4 weeks, arranged in eight separate groups.
Reconstituted onabotulinumtoxinA (2.5 U/0.1 mL) was placed securely in a battery-operated mechanized apparatus. The apparatus with the vial was placed in the refrigerator, and the apparatus continuously inverted and straightened the onabotulinumtoxinA vial 30 times a minute. Eight mice were each injected intraperitoneally with 1 U of the agitated onabotulinumtoxinA on days 1, 3, 5, 7, 14, 21, 28, and 42, to evaluate the efficacy of the continuously agitated onabotulinumtoxinA.
Death of the mice, demonstrating efficacy of the onabotulinumtoxinA molecule.
Half of each group of mice (4/8 mice) died within 48 hours of the injection (range 16-48 hours).
The effect of botulinum toxin type A is maintained even when it is agitated vigorously for up to 6 weeks. The authors have indicated no significant interest with commercial supporters.
肉毒毒素 A 被认为是一种极其脆弱的分子，一些已配制的肉毒毒素 A 制剂建议在配制后 4 小时内使用。建议极度小心，避免摇晃小瓶。
确定即使剧烈摇晃，已配制的肉毒毒素 A 的效果是否仍然保持。
实验性、对照、动物研究。
64 只 2 至 4 周龄的白色瑞士 Webster 小鼠，分为 8 个单独的组。
将已配制的肉毒毒素 A（2.5U/0.1mL）牢固地放置在电池供电的机械化仪器中。将仪器和小瓶放入冰箱中，仪器每分钟将装有肉毒毒素 A 的小瓶连续翻转和拉直 30 次。将 8 只小鼠分别在第 1、3、5、7、14、21、28 和 42 天经腹腔注射 1U 剧烈搅拌的肉毒毒素 A，以评估连续搅拌的肉毒毒素 A 的疗效。
小鼠死亡，证明肉毒毒素 A 分子的疗效。
每组小鼠的一半（4/8 只）在注射后 48 小时内死亡（范围 16-48 小时）。
即使剧烈搅拌长达 6 周，肉毒毒素 A 型的效果仍然保持。作者表示没有与商业支持者的重大利益关系。
