Department of Epidemiology and Public Health, Leahurst, University of Liverpool, Liverpool, UK.
Epidemiol Infect. 2011 Nov;139(11):1661-71. doi: 10.1017/S0950268810002736. Epub 2010 Dec 7.
Multi-locus sequence typing was performed on 1003 Campylobacter jejuni isolates collected in a 2-year longitudinal study of 15 dairy farms and four sheep farms in Lancashire, UK. There was considerable farm-level variation in occurrence and prevalence of clonal complexes (CC). Clonal complexes ST61, ST21, ST403 and ST45 were most prevalent in cattle while in sheep CC ST42, ST21, ST48 and ST52 were most prevalent. CC ST45, a complex previously shown to be more common in summer months in human cases, was more prevalent in summer in our ruminant samples. Gene flow analysis demonstrated a high level of genetic heterogeneity at the within-farm level. Sequence-type diversity was greater in cattle compared to sheep, in cattle at pasture vs. housed, and in isolates from farms on the Pennines compared to the Southern Fylde. Sequence-type diversity was greatest in isolates belonging to CC ST21, ST45 and ST206.
对英国兰开夏郡 15 家奶牛场和 4 家绵羊场进行为期 2 年的纵向研究中收集的 1003 株空肠弯曲菌分离株进行了多位点序列分型。克隆复合体（CC）的发生和流行存在很大的农场水平差异。克隆复合体 ST61、ST21、ST403 和 ST45 在牛中最为流行，而在绵羊中 CC ST42、ST21、ST48 和 ST52 最为流行。CC ST45 是一种先前在人类病例中夏季更为常见的复杂型，在我们的反刍动物样本中，夏季更为流行。基因流动分析表明，农场内的遗传异质性水平很高。与绵羊相比，牛的序列型多样性更大，放牧牛比圈养牛更大，奔宁山脉农场的分离株比南菲尔兹农场更大。属于 CC ST21、ST45 和 ST206 的分离株的序列型多样性最大。
