Saravanan Nadana, Nalini Namasivayam
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India.
J Med Food. 2007 Dec;10(4):675-82. doi: 10.1089/jmf.2006.110.
The antioxidant effect of the ethanolic root extract of Hemidesmus indicus, an indigenous Ayurvedic medicinal plant used in soft drinks in India, was studied in rats with ethanol-induced hepatotoxicity. Administering 20% ethanol (5 g/kg of body weight/day) for 60 days to male Wistar rats resulted in significantly decreased body weight and increased liver/body weight ratio. The liver marker enzymes, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatae (ALP), gamma-glutamyl transpeptidase (GGT), and lactate dehydrogenase (LDH), were elevated. In addition, the levels of plasma, erythrocyte, and hepatic thiobarbituric acid-reactive substances (TBARS), hydroperoxides (LOOH), and conjugated dienes (CD) were also elevated in ethanol-fed rats as compared to those of the experimental control rats. Decreased activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), vitamin C, and alpha-tocopherol (vitamin E) were also observed in ethanol-administered as compared to control rats. Ethanolic root extract of H. indicus was administered at a dose of 500 mg/kg of body weight/day for the last 30 days of the experiment to rats with ethanol-induced liver injury, which significantly increased body weight, significantly decreased the liver/body weight ratio, AST, ALT, ALP, GGT, and LDH activities, and also the levels of TBARS, LOOH, and CD, significantly elevated the activities of SOD, CAT, GPx, and GSH in plasma, erythrocytes, and liver, and also increased levels of plasma and liver vitamin C and vitamin E at the end of the experimental period as compared to those of untreated ethanol-administered rats. Thus, our data indicate that treatment with H. indicus extract offers protection against free radical-mediated oxidative stress in plasma, erythrocytes, and liver of animals with ethanol-induced liver injury.
印度一种本土阿育吠陀药用植物印度牛弥菜的乙醇根提取物的抗氧化作用，在乙醇诱导肝毒性的大鼠中进行了研究。给雄性Wistar大鼠连续60天每天给予20%乙醇（5克/千克体重），导致体重显著下降，肝/体重比增加。肝脏标志物酶，天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）、γ-谷氨酰转肽酶（GGT）和乳酸脱氢酶（LDH）升高。此外，与实验对照大鼠相比，乙醇喂养大鼠的血浆、红细胞和肝脏硫代巴比妥酸反应性物质（TBARS）、氢过氧化物（LOOH）和共轭二烯（CD）水平也升高。与对照大鼠相比，在给予乙醇的大鼠中还观察到超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、还原型谷胱甘肽（GSH）、维生素C和α-生育酚（维生素E）的活性降低。在实验的最后30天，给乙醇诱导肝损伤的大鼠每天按500毫克/千克体重的剂量给予印度牛弥菜乙醇根提取物，这显著增加了体重，显著降低了肝/体重比、AST、ALT、ALP、GGT和LDH活性，以及TBARS、LOOH和CD水平，显著提高了血浆、红细胞和肝脏中SOD、CAT、GPx和GSH的活性，并且在实验期结束时与未处理的乙醇喂养大鼠相比，血浆和肝脏中的维生素C和维生素E水平也增加。因此，我们的数据表明，用印度牛弥菜提取物治疗可为乙醇诱导肝损伤动物的血浆、红细胞和肝脏中自由基介导的氧化应激提供保护。
