Department of Human Biology, Nutrition and Toxicology, Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
Proteomics Clin Appl. 2007 Oct;1(10):1306-15. doi: 10.1002/prca.200700049. Epub 2007 Sep 11.
Differences in fat metabolism are of importance in relation to energy balance. Low fat-oxidizers (LFO) are thought to be more prone for developing obesity. We studied whether LFO have different fasting adipose tissue (AT) protein profiles than high fat-oxidizers (HFO). Six LFO and six HFO subjects were selected from an obese group (n = 99, body mass index>30 kg/m(2) ) taking part in a multi-center study (Nutrient-Gene interaction in human obesity) based on the postprandial fat oxidation capacity after a high fat load. AT protein profiles were studied by 2-DE. Differential proteins were clustered with MAPPfinder according to their function. Protein profiles of purified blood cells and adipocytes served to confine the comparison to adipocyte-specific proteins in AT profiles of LFO and HFO subjects. LFO had increased mitochondrial ROS scavengers possibly related to long-chain unsaturated fatty acid-induced increases in mitochondrial ROS-production. Carbohydrate oxidation seemed to be reduced since expression of several proteins from the glycolysis pathway was lower in LFO. Up-regulation of the valine catabolism at the level of methylmalonate-semialdehyde dehydrogenase appeared to be (part of) the compensatory mechanism. In conclusion, the fasting AT protein profile of LFO and HFO differ at the level of ROS scavenging, the glycolysis pathway and valine metabolism.
脂肪代谢的差异与能量平衡有关。低脂肪氧化者（LFO）被认为更容易发展为肥胖。我们研究了 LFO 是否与高脂肪氧化者（HFO）相比，其空腹脂肪组织（AT）蛋白图谱存在差异。从一项肥胖症多中心研究（基于高脂肪负荷后餐后脂肪氧化能力）中选择了 6 名 LFO 和 6 名 HFO 受试者（Nutrient-Gene interaction in human obesity），该研究共纳入 99 名受试者（BMI>30 kg/m(2)）。通过 2-DE 研究 AT 蛋白图谱。根据功能，用 MAPPfinder 将差异蛋白进行聚类。纯化的血细胞和脂肪细胞的蛋白图谱用于将比较限制在 LFO 和 HFO 受试者的 AT 图谱中的脂肪细胞特异性蛋白。LFO 可能增加了线粒体 ROS 清除剂，这可能与长链不饱和脂肪酸引起的线粒体 ROS 产生增加有关。由于糖酵解途径的几种蛋白质表达较低，碳水化合物氧化似乎减少。缬氨酸分解代谢的上调（部分）似乎是（补偿机制的）一部分。结论：LFO 和 HFO 的空腹 AT 蛋白图谱在 ROS 清除、糖酵解途径和缬氨酸代谢水平上存在差异。
