Cancer Research UK Clinical Centre, St. James's University Hospital, University of Leeds, Leeds, UK.
Proteomics Clin Appl. 2007 Aug;1(8):739-46. doi: 10.1002/prca.200700380. Epub 2007 Jul 10.
Although proteomic technologies have been enthusiastically embraced in the field of biomarker discovery and particularly with biological fluids, it is increasingly being recognized that pre-analytical effects, i.e. those occurring prior to the point of actual sample analysis and including factors such as sample processing and storage, can exert marked influences on the results obtained. Such effects have been recognized already for specific analytes in clinical chemistry, but with the increasing sensitivity and resolution of the newer technologies, such effects are potentially even more marked. The challenge of translating initial findings into clinical application requires such issues to be addressed at a very early stage in study design and this paper reviews the current knowledge of the potential impact of pre-analytical factors on proteomic studies of biological fluids.
尽管蛋白质组学技术在生物标志物发现领域得到了广泛应用，特别是在生物体液方面，但人们越来越认识到，分析前效应（即在实际样本分析之前发生的影响，包括样本处理和储存等因素）会对所获得的结果产生显著影响。这种影响已经在临床化学的特定分析物中得到了认识，但随着新技术的灵敏度和分辨率的提高，这种影响可能更为显著。将初步发现转化为临床应用的挑战要求在研究设计的早期阶段解决这些问题，本文综述了分析前因素对生物体液蛋白质组学研究的潜在影响的现有知识。
