Department of Pathology, University of Washington School of Medicine, Seattle, WA, USA.
Proteomics Clin Appl. 2008 Oct;2(10-11):1484-97. doi: 10.1002/prca.200800043. Epub 2008 Sep 10.
Proteomics has revealed itself as a powerful tool in the identification and determination of proteins and their biological significance. More recently, several groups have taken advantage of the high-throughput nature of proteomics in order to gain a more in-depth understanding of the human brain. In turn, this information has provided researchers with invaluable insight into the potential pathways and mechanisms involved in the pathogenesis of several neurodegenerative disorders, e.g., Alzheimer and Parkinson disease. Furthermore, these findings likely will improve methods to diagnose disease and monitor disease progression as well as generate novel targets for therapeutic intervention. Despite these advances, comprehensive understanding of the human brain proteome remains challenging, and requires development of improved sample enrichment, better instrumentation, and innovative analytic techniques. In this review, we will focus on the most recent progress related to identification of proteins in the human brain under normal as well as pathological conditions, mainly Alzheimer and Parkinson disease, their potential application in biomarker discovery, and discuss current advances in protein identification aimed at providing a more comprehensive understanding of the brain.
蛋白质组学已成为鉴定蛋白质及其生物学意义的有力工具。最近，一些研究小组利用蛋白质组学的高通量特性，以更深入地了解人类大脑。反过来，这些信息为研究人员提供了有价值的见解，了解几种神经退行性疾病（如阿尔茨海默病和帕金森病）发病机制中涉及的潜在途径和机制。此外，这些发现可能会改进疾病诊断和监测疾病进展的方法，并为治疗干预产生新的靶点。尽管取得了这些进展，但全面了解人类大脑蛋白质组仍然具有挑战性，需要开发改进的样品富集、更好的仪器和创新的分析技术。在这篇综述中，我们将重点介绍与正常和病理条件下（主要是阿尔茨海默病和帕金森病）人类大脑蛋白质的鉴定以及它们在生物标志物发现中的潜在应用相关的最新进展，并讨论旨在更全面地了解大脑的蛋白质鉴定方面的当前进展。
