School of Biological Sciences, University of Wollongong, Wollongong, New South Wales, Australia.
Proteomics Clin Appl. 2008 Mar;2(3):387-410. doi: 10.1002/prca.200780048.
Gram-positive streptococci are non-motile, chain-forming bacteria commonly found in the normal oral and bowel flora of warm-blooded animals. Over the past decade, a proteomic approach combining 2-DE and MS has been used to systematically map the cellular, surface-associated and secreted proteins of human pathogenic streptococcal species. The public availability of complete streptococcal genomic sequences and the amalgamation of proteomic, genomic and bioinformatic technologies have recently facilitated the identification of novel streptococcal vaccine candidate antigens and therapeutic agents. The objective of this review is to examine the constituents of the streptococcal cell wall and secreted proteome, the mechanisms of transport of surface and secreted proteins, and describe the current methodologies employed for the identification of novel surface-displayed proteins and potential vaccine antigens.
革兰氏阳性链球菌是无动力、链状形成的细菌，通常存在于温血动物的正常口腔和肠道菌群中。在过去的十年中，一种结合了 2-DE 和 MS 的蛋白质组学方法被用于系统地绘制人类致病性链球菌物种的细胞、表面相关和分泌蛋白图谱。完整的链球菌基因组序列的公开可用性以及蛋白质组学、基因组学和生物信息学技术的融合，最近促进了新型链球菌疫苗候选抗原和治疗剂的鉴定。本综述的目的是检查链球菌细胞壁和分泌蛋白质组的组成、表面和分泌蛋白的运输机制，并描述目前用于鉴定新型表面展示蛋白和潜在疫苗抗原的方法。
