Department of Physiology and Biophysics and Center for Cardiovascular Research, University of Illinois at Chicago, Chicago, IL, USA.
Proteomics Clin Appl. 2008 Jun;2(6):788-99. doi: 10.1002/prca.200780076.
Myofilament proteins of the cardiac sarcomere house the molecular machinery responsible for generating tension and pressure. Release of intracellular Ca(2+) triggers myofilament tension generation and shortening, but the response to Ca(2+) is modulated by changes in key regulatory proteins. We review how these proteomic changes are essential to adaptive physiological regulation of cardiac output and become maladaptive in cardiac disorders. We also review the essentials of proteomic techniques used to study myofilament protein changes, including degradation, isoform expression, phosphorylation and oxidation. Selected proteomic studies illustrate the applications of these approaches.
心肌细胞肌节中的肌丝蛋白构成了产生张力和压力的分子机制。细胞内 Ca(2+) 的释放触发肌丝张力的产生和缩短，但 Ca(2+) 的反应受到关键调节蛋白变化的调节。我们回顾了这些蛋白质组变化如何对心脏输出的适应性生理调节至关重要，并在心脏疾病中变得适应不良。我们还回顾了用于研究肌丝蛋白变化的蛋白质组技术的要点，包括降解、同工型表达、磷酸化和氧化。选定的蛋白质组学研究说明了这些方法的应用。
