[高效液相色谱法测定医用海龙中胸腺嘧啶、次黄嘌呤和尿嘧啶含量]

[Determination of thymine, hypoxanthine and uracil contents in medical pipefish by HPLC].

作者信息

Wang Mengyue, Wei Jingfei, Shi Haiming, Li Xiaobo

机构信息

School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Sep;35(17):2277-80.

PMID:21137337

Abstract

OBJECTIVE

To establish a RP-HPLC method for simultaneous determination of thymine, hypoxanthine and uracil contents in medical pipefish.

METHOD

Samples were extracted with distilled water by ultrasonic wave and separated on Waters C18 column eluted with a mobile phase of 0.05 mol x L(-1) KFI2PO4-acetonitrile (97:3). The flow rate was 0.6 mL x min(-1). The determination wavelength was 260 nm and the column temperature was set at 40 degrees C.

RESULT

The method had good linearity in the range of 0.033-0.660 (r = 0.9996), 0.620-12.400 (r = 0.9999), 0.048-0.960 microg (r = 0.9995), with average recoveries of 98.67% (RSD 1.6%), 99.03% (RSD 0.74%), 98.65% (RSD 1.8%), for thymine, hypoxanthine and uracil respectively.

CONCLUSION

The simultaneous determination method of thymine, hypoxanthine and uracil in medical pipefish is established by RP-HPLC for the first time. The contents of the three constituents in different kinds of medical pipefish are significantly different. The method is simple, rapid and sensitive, and can be used for control the quality of medical pipefish.

摘要

目的

建立一种反相高效液相色谱法（RP-HPLC）同时测定药用海龙中胸腺嘧啶、次黄嘌呤和尿嘧啶的含量。

方法

样品用蒸馏水超声提取，在Waters C18柱上分离，流动相为0.05 mol·L⁻¹ KH₂PO₄-乙腈（97:3），流速为0.6 mL·min⁻¹，测定波长为260 nm，柱温设定为40℃。

结果

该方法在0.033 - 0.660（r = 0.9996）、0.620 - 12.400（r = 0.9999）、0.048 - 0.960 μg（r = 0.9995）范围内线性良好，胸腺嘧啶、次黄嘌呤和尿嘧啶的平均回收率分别为98.67%（RSD 1.6%）、99.03%（RSD 0.74%）、98.65%（RSD 1.8%）。