Bai Cheng-Ke, Wen Miao-Miao, Yu Feng, Li Gui-Shuang
Shaanxi Normal University, College of Life Sciences, China.
Zhong Yao Cai. 2010 Nov;33(11):1689-94.
To build up primary core germplasm of Scutellaria baicalensis.
The genetic diversity of 40 germplasm resources of Scutellaria baicalensis in different province were analyzed by ISSR, and the primary core germplasm were constructed with progressive sampling method of smallest genetic distance.
15 primers, which showed good repetitive, special bands and distinct polymorphism, were selected from 51 random ISSR primers. Then the total 248 loci were amplified by these selected 15 primers, with a 97.17% polymorphic loci. The average of Shannon information index (I), Nei's genetic diversity (H), number of alleles and effective number of alleles (NE) by POPGENE 32 analysis were 0.4353, 0.2819, 1.9640 and 1.4617, respectively. It showed there was highly genetic diversity in the 40 germplasm resources. The result of analysis by NTSYS-PC software shows the genetic similarity (Gs) were among 0.64 and 0.80, and there was upper coherence between the clustering result and source core germplasm collection except individual germplasms. The result showed the percentage of polymorphic loci was obviously reduced and the Shannon's information index and Nei's genetic diversity were increased a little, but the index change of germplasm genetic diversity was less than that before sampling. The core germplasms from No. 3 sampling were most representative, whose sampling number was about 30% of the initial sampling, and the percentage of polymorphic loci was that of before sampling 96.8%.
It was practicable that the methods would be used to construct core germplasm collection of Scutellaria baicalensis by ISSR marker.
构建黄芩初级核心种质。
采用ISSR技术对来自不同省份的40份黄芩种质资源的遗传多样性进行分析，并采用最小遗传距离逐步取样法构建初级核心种质。
从51条随机ISSR引物中筛选出15条重复性好、条带特异、多态性明显的引物。这15条引物共扩增出248个位点，多态性位点百分率为97.17%。利用POPGENE 32软件分析，Shannon信息指数（I）、Nei's遗传多样性（H）、等位基因数和有效等位基因数（NE）的平均值分别为0.4353、0.2819、1.9640和1.4617，表明40份黄芩种质资源具有较高的遗传多样性。NTSYS-PC软件分析结果显示，遗传相似系数（Gs）在0.64至0.80之间，除个别种质外，聚类结果与来源核心种质库之间具有较高的一致性。结果表明，多态性位点百分率明显降低，Shannon信息指数和Nei's遗传多样性略有增加，但种质遗传多样性指数变化小于取样前。第3次取样得到的核心种质最具代表性，取样数量约为初始取样的30%，多态性位点百分率为取样前的96.8%。
利用ISSR标记构建黄芩核心种质库的方法可行。
