Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Biomacromolecules. 2011 Jan 10;12(1):14-8. doi: 10.1021/bm101246u. Epub 2010 Dec 7.
We report a facile method to fabricate free-standing, 3D hydrogel microarchitectures of chemically sol-gel transitional hydrogels, which is based on the use of hydrophilic substrate and aerosol of gelling agent without molding (or sandwiching) process. Using proposed methods, we fabricated hydrogel microarchitectures of sheets, meshes, or microunits without morphological distortions on the microscale. These hydrogel microarchitectures could be easily and stably exfoliated from the substrates and cultured (in the case of containing cells). These free-standing hydrogel microarchitectures in sheets, meshes, or microunits can be easily harvested and assembled as a biofabrication unit to generate complex composites with controlled microscale structures.
我们报告了一种制备独立的 3D 水凝胶微结构的简便方法，该方法基于使用亲水基底和雾化的凝胶剂，而无需成型（或夹层）工艺。使用所提出的方法，我们在微尺度上制造了没有形态变形的片状、网状或微单元水凝胶微结构。这些水凝胶微结构可以很容易地从基底上剥离并进行培养（如果含有细胞的话）。这些独立的片状、网状或微单元水凝胶微结构可以很容易地收获和组装，作为一个生物制造单元，以生成具有受控微尺度结构的复杂复合材料。
