Breidt F, Dubnau D
Department of Microbiology, Public Health Research Institute, New York, New York 10016.
J Bacteriol. 1990 Jul;172(7):3661-8. doi: 10.1128/jb.172.7.3661-3668.1990.
ermC methylase gene expression has been shown to be limited by translational autorepression, presumably due to methylase binding to ermC mRNA. It was found that this repression occurs in trans, yielding a 50% reduction in translation of an ermC-lacZ fusion mRNA. We investigated the ermC mRNA sequences required for translational repression in vivo. A series of deletions identified sequences in the 5' regulatory region that were required for translational repression. These included sequences of the 5' stem-loop structure that were not required for induction, as well as some that were required. The implications of these results for regulation are discussed.
ermC甲基化酶基因的表达已被证明受翻译自抑制的限制，推测是由于甲基化酶与ermC mRNA结合所致。研究发现这种抑制以反式作用发生，导致ermC - lacZ融合mRNA的翻译减少50%。我们研究了体内翻译抑制所需的ermC mRNA序列。一系列缺失鉴定出5'调控区域中翻译抑制所需的序列。这些序列包括诱导所不需要的5'茎环结构的序列，以及一些所需的序列。讨论了这些结果对调控的意义。
