New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Curr Microbiol. 2011 Apr;62(4):1123-7. doi: 10.1007/s00284-010-9831-y. Epub 2010 Dec 8.
The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.
氧化葡萄糖酸杆菌 M5 中吡咯喹啉醌依赖的膜结合型醛脱氢酶（ALDH）被敲除后，可用于苯甲醇的氧化。工程菌株对苯甲醛的选择性明显提高，达到 67.3%，而野生菌株仅有 2.8%。同时，水/异辛烷（1:1）两相体系进一步提高了选择性。通过这些方法，在最适初始苯甲醇浓度为 5.0 g/L 时，在 1 h 内可几乎达到 100%的选择性和转化率。
