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Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose.氧化葡萄糖酸杆菌M5中负责生产6-(2-羟乙基)氨基-6-脱氧-L-山梨糖的膜结合吡咯喹啉醌依赖性脱氢酶。
Appl Environ Microbiol. 2008 Aug;74(16):5250-3. doi: 10.1128/AEM.00272-08. Epub 2008 May 23.
2
Synergistic improvement of PQQ-dependent D-sorbitol dehydrogenase activity from Gluconobacter oxydans for the biosynthesis of miglitol precursor 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose.协同提高氧化葡萄糖酸杆菌依赖吡咯喹啉醌的 D-山梨醇脱氢酶活性，用于合成米格列醇前体 6-（N-羟乙基）-氨基-6-脱氧-α-L-山梨呋喃糖。
J Biotechnol. 2019 Jul 20;300:55-62. doi: 10.1016/j.jbiotec.2019.05.007. Epub 2019 May 14.
3
Combinational expression of D-sorbitol dehydrogenase and pyrroloquinoline quinone increases 6-(N-hydroxyethyl)-amino-6-deoxy-α-L-sorbofuranose production by Gluconobacter oxydans through cofactor manipulation.通过辅酶操纵，氧化葡萄糖酸杆菌中 D-山梨醇脱氢酶和吡咯喹啉醌的组合表达增加了 6-（N-羟乙基）-氨基-6-脱氧-α-L-山梨呋喃糖的产量。
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A pyrroloquinoline quinine-dependent membrane-bound d-sorbitol dehydrogenase from Gluconobacter oxydans exhibits an ordered Bi Bi reaction mechanism.氧化葡萄糖酸杆菌中一种依赖于吡咯喹啉醌的膜结合d-山梨醇脱氢酶呈现有序的双底物双产物反应机制。
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A highly efficient sorbitol dehydrogenase from Gluconobacter oxydans G624 and improvement of its stability through immobilization.一株氧化葡萄糖酸杆菌 G624 来源的高效山梨醇脱氢酶及其固定化提高其稳定性。
Sci Rep. 2016 Sep 16;6:33438. doi: 10.1038/srep33438.
7
Enhanced production of L-sorbose from D-sorbitol by improving the mRNA abundance of sorbitol dehydrogenase in Gluconobacter oxydans WSH-003.通过提高氧化葡萄糖酸杆菌WSH-003中山梨醇脱氢酶的mRNA丰度增强从D-山梨醇生产L-山梨糖。
Microb Cell Fact. 2014 Oct 18;13:146. doi: 10.1186/s12934-014-0146-8.
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Molecular properties of membrane-bound FAD-containing D-sorbitol dehydrogenase from thermotolerant Gluconobacter frateurii isolated from Thailand.从泰国分离出的耐热弗氏葡萄糖杆菌中膜结合含黄素腺嘌呤二核苷酸的D-山梨醇脱氢酶的分子特性
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Optimized synthesis of L-sorbose by C(5)-dehydrogenation of D-sorbitol with Gluconobacter oxydans.利用氧化葡萄糖酸杆菌通过D-山梨醇的C(5)脱氢优化合成L-山梨糖。
Biotechnol Bioeng. 2000 Aug 5;69(3):339-43. doi: 10.1002/1097-0290(20000805)69:3<339::aid-bit12>3.0.co;2-e.

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Characterization of a novel NADPH-dependent oxidoreductase from Gluconobacter oxydans.从氧化葡萄糖酸杆菌中鉴定一种新型的 NADPH 依赖型氧化还原酶。
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本文引用的文献

1
The genus Gluconobacter oxydans: comprehensive overview of biochemistry and biotechnological applications.氧化葡萄糖杆菌属：生物化学与生物技术应用综述
Crit Rev Biotechnol. 2007 Jul-Sep;27(3):147-71. doi: 10.1080/07388550701503584.
2
Asymmetric oxidation by Gluconobacter oxydans.氧化葡萄糖酸杆菌的不对称氧化作用。
Appl Microbiol Biotechnol. 2006 Mar;70(2):135-9. doi: 10.1007/s00253-005-0307-0. Epub 2006 Jan 24.
3
Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans.氧化葡萄糖酸杆菌的全基因组序列
Nat Biotechnol. 2005 Feb;23(2):195-200. doi: 10.1038/nbt1062. Epub 2005 Jan 23.
4
Cloning of a gluconate/polyol dehydrogenase gene from Gluconobacter suboxydans IFO 12528, characterisation of the enzyme and its use for the production of 5-ketogluconate in a recombinant Escherichia coli strain.从弱氧化葡糖杆菌IFO 12528中克隆葡萄糖酸盐/多元醇脱氢酶基因、对该酶进行表征及其在重组大肠杆菌菌株中用于生产5-酮基葡萄糖酸盐的应用
Appl Microbiol Biotechnol. 2004 Aug;65(3):306-14. doi: 10.1007/s00253-004-1594-6. Epub 2004 Apr 2.
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A new method for rapidly generating gene-targeting vectors by engineering BACs through homologous recombination in bacteria.一种通过细菌中的同源重组对细菌人工染色体（BAC）进行工程改造来快速生成基因靶向载体的新方法。
Genome Res. 2003 Sep;13(9):2190-4. doi: 10.1101/gr.1356503. Epub 2003 Aug 12.
6
Membrane-bound D-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255--enzymatic and genetic characterization.氧化葡萄糖酸杆菌IFO 3255的膜结合D-山梨醇脱氢酶——酶学及遗传学特性研究
Biochim Biophys Acta. 2003 Apr 11;1647(1-2):278-88. doi: 10.1016/s1570-9639(03)00071-2.
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5-keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in gluconobacter species.5-酮基-D-葡萄糖酸盐的产生由葡萄糖酸杆菌属中的一种醌蛋白甘油脱氢酶（主要的多元醇脱氢酶）催化。
Appl Environ Microbiol. 2003 Apr;69(4):1959-66. doi: 10.1128/AEM.69.4.1959-1966.2003.
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New developments in oxidative fermentation.氧化发酵的新进展。
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9
Main polyol dehydrogenase of Gluconobacter suboxydans IFO 3255, membrane-bound D-sorbitol dehydrogenase, that needs product of upstream gene, sldB, for activity.弱氧化葡糖杆菌IFO 3255的主要多元醇脱氢酶，即膜结合D-山梨醇脱氢酶，其活性需要上游基因sldB的产物。
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10
Biochemistry and biotechnological applications of Gluconobacter strains.葡萄糖酸杆菌菌株的生物化学与生物技术应用
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氧化葡萄糖酸杆菌M5中负责生产6-(2-羟乙基)氨基-6-脱氧-L-山梨糖的膜结合吡咯喹啉醌依赖性脱氢酶。

Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose.

作者信息

Yang Xue-Peng, Wei Liu-Jing, Lin Jin-Ping, Yin Bo, Wei Dong-Zhi

机构信息

State Key Laboratory of Bioreactor Engineering, East China University of Science and Biotechnology, Mei-Long 130, Shanghai 200237, China.

出版信息

Appl Environ Microbiol. 2008 Aug;74(16):5250-3. doi: 10.1128/AEM.00272-08. Epub 2008 May 23.

DOI:10.1128/AEM.00272-08
PMID:18502922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2519276/
Abstract

A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.

摘要

从氧化葡萄糖酸杆菌M5中纯化得到的一种膜结合蛋白被证实为吡咯喹啉醌依赖性D-山梨醇脱氢酶。基因敲除和互补实验表明，该酶负责将1-(2-羟乙基)氨基-1-脱氧-D-山梨醇（1NSL）氧化为6-(2-羟乙基)氨基-6-脱氧-L-山梨糖（6NSE），而6NSE是抗糖尿病药物米格列醇的前体。