School of Chemical and Biological Engineering, Seoul National University, Seoul, Republic of Korea.
Biotechnol Lett. 2011 Apr;33(4):845-51. doi: 10.1007/s10529-010-0492-5. Epub 2010 Dec 8.
Fibroblast growth factor 2 (FGF2) stimulates skin wound healing but does long-term delivery of FGF2 enhance skin regeneration compared to short-term delivery? Heparin-conjugated fibrin (HCF) was used as a vehicle for long-term delivery of FGF2. Fibrin, HCF, FGF2-loaded fibrin, and FGF2-loaded HCF were implanted into full-thickness skin defects of mice. The neoepidermis thickness was significantly larger in the FGF2-loaded HCF group than in the other groups, except for the FGF2-loaded fibrin group. Suprabasal cytokeratin differentiation in squamous neoepithelium was greatest in the FGF2-loaded HCF group. The enhanced skin regeneration accompanying the long-term delivery of FGF2 could be mediated, at least partially, by enhanced neovascularization and cell proliferation in the neodermis.
成纤维细胞生长因子 2（FGF2）可刺激皮肤伤口愈合，但与短期递送相比，长期递送 FGF2 是否会增强皮肤再生？肝素结合纤维蛋白（HCF）被用作 FGF2 的长期递送载体。将纤维蛋白、HCF、负载 FGF2 的纤维蛋白和负载 FGF2 的 HCF 植入小鼠的全层皮肤缺损中。与其他组相比，除了负载 FGF2 的纤维蛋白组之外，负载 FGF2 的 HCF 组的新生表皮厚度明显更大。在负载 FGF2 的 HCF 组中，鳞状新生上皮的细胞角蛋白超顶分化最大。伴随 FGF2 长期递送的增强的皮肤再生至少部分可以通过真皮中的新生血管形成和细胞增殖来介导。
