Huang Yan, Yang Sheng, Zhou Wen-Xia, Zhang Yong-Xiang
Beijing Institute of Pharmacology and Toxicology, Beijing 100850, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):291-5.
To establish the method of long-term potentiation (LTP) recording in hippocampus in anaesthetized mice in vivo.
Mouse was anaesthetized and then placed in the stereotaxic apparatus. The recording electrode was located at the cell body layer of dentate granule cells and the stimulating electrode at the perforant path according to stereotaxic parameters. Then the LTP was evoked and recorded.
After optimizing of experimental factor, the LTP in PP-DG path in anaesthetized Balb/c mice was successfully recorded. The changes of synaptic plasticity were also observed in SAMP8 and SAMRI by using the optimized method. The results were coincident with the behavioral tests and LTP in hippocampal slices that we reported before.
The method of LTP recording in vivo in hippocampus in anaesthetized mice was successfully established and could be used to evaluate the synaptic plasticity in vivo.
建立在麻醉小鼠体内海马长时程增强（LTP）记录的方法。
将小鼠麻醉后置于立体定位仪中。根据立体定位参数，记录电极置于齿状颗粒细胞的胞体层，刺激电极置于穿通通路。然后诱发并记录LTP。
优化实验因素后，成功记录了麻醉的Balb/c小鼠穿通通路-齿状颗粒细胞（PP-DG）通路中的LTP。使用优化方法在SAMP8和SAMRI中也观察到了突触可塑性的变化。结果与我们之前报道的行为测试和海马脑片LTP一致。
成功建立了麻醉小鼠体内海马LTP记录方法，可用于评估体内突触可塑性。
