Zhu Yan, Zhou Ai-Ling, Mao Jia-Hui, Hu Ya-E, Shi Hai-Yan
Department of Pathophysiology, School of Medical Sciences, Nantong University, Nantong 226001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):296-300.
To investigate the mechanisms of Naoyikang (Traditional Chinese Medicine) on the Alzheimer's Disease (AD) model mice induced by D-galactose (D-gal) and NaNO2.
The mouse model was established by intraperitoneal injection of D-gal and NaNO2. The capacity of learning and memory was tested on mice with electrical maze; the content of nitric oxide (NO) and the activity of monoamine oxidase-B (MAO-B), glutathione peroxidase (GSH-PX), Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme in cerebral cortex and hippocampus were assayed by biochemical methods; expression of Bax and Bcl-2 mRNA was detested by RT-PCR.
Naoyikang could ameliorate the capacity of learning and memory of AD model mice and reduce MAO-B activity in the brain tissue and activate the activity of Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme in the brain tissue and decrease the expression of Bax mRNA, but increase the expression of Bcl-2 mRNA in the model brain tissue.
Naoyikang could protect AD model mice induced by D-gal and NaNO2. It could modify the metabolism of monoamine neurotransmitter in brain through reducing MAO-B activity and protect neurons by activating the activity of Na(+) -K(+) -ATP enzyme and Ca(2+) -ATP enzyme and decrease Bax expression and increase Bcl-2 expression in the model brain tissue.
探讨脑益康（中药）对D-半乳糖（D-gal）和亚硝酸钠诱导的阿尔茨海默病（AD）模型小鼠的作用机制。
通过腹腔注射D-半乳糖和亚硝酸钠建立小鼠模型。用电迷宫测试小鼠的学习记忆能力；采用生化方法检测大脑皮质和海马中一氧化氮（NO）含量、单胺氧化酶B（MAO-B）、谷胱甘肽过氧化物酶（GSH-PX）、钠钾ATP酶（Na(+) -K(+) -ATP酶）和钙ATP酶（Ca(2+) -ATP酶）的活性；用逆转录聚合酶链反应（RT-PCR）检测Bax和Bcl-2 mRNA的表达。
脑益康可改善AD模型小鼠的学习记忆能力，降低脑组织中MAO-B活性，激活脑组织中Na(+) -K(+) -ATP酶和Ca(2+) -ATP酶的活性，降低模型脑组织中Bax mRNA的表达，但增加Bcl-2 mRNA的表达。
脑益康对D-半乳糖和亚硝酸钠诱导的AD模型小鼠具有保护作用。它可通过降低MAO-B活性来调节脑内单胺类神经递质的代谢，通过激活Na(+) -K(+) -ATP酶和Ca(2+) -ATP酶的活性保护神经元，并降低模型脑组织中Bax的表达，增加Bcl-2的表达。
