Zhong Shu-Zhi, Ge Qing-Hua, Qu Rong, Li Qiao, Ma Shi-Ping
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing, 210038, China.
J Neurol Sci. 2009 Feb 15;277(1-2):58-64. doi: 10.1016/j.jns.2008.10.008. Epub 2008 Nov 12.
In the present study, we examined the supplementation of paeonol extracted from Moutan cortex of Paeonia suffruticosa Andrews (MC) or the root of Paeonia lactiflora Pall (PL) on reducing oxidative stress, cognitive impairment and neurotoxicity in d-galactose (D-gal)-induced aging mice. The ICR mice were subcutaneously injected with D-gal (50 mg/(kg day)) for 60 days and administered with paeonol (50, 100 mg/(kg day)) simultaneously. The results showed that paeonol significantly improved the learning and memory ability in Morris water maze test and step-down passive avoidance test in D-gal-treated mice. Further investigation showed that the effect of paeonol on improvement of cognitive deficit was related to its ability to inhibit the biochemical changes in brains of D-gal-treated mice. Paeonol increased acetylcholine (Ach) and glutathione (GSH) levels, restored superoxide dismutase (SOD) and Na(+), K(+)-adenosine triphosphatase (Na(+), K(+)-ATPase) activities, but decreased cholinesterase AChe activity and malondialdehyde (MDA) level in D-gal-treated mice. Furthermore, paeonol ameliorated neuronal damage in both hippocampus and temporal cortex in D-gal-treated mice. These results suggest that paeonol possesses anti-aging efficacy and may have potential in treatment of neurodegenerative diseases.
在本研究中，我们检测了从牡丹皮（MC）或芍药根（PL）中提取的丹皮酚对减轻D-半乳糖（D-gal）诱导的衰老小鼠氧化应激、认知障碍和神经毒性的作用。将ICR小鼠皮下注射D-半乳糖（50mg/（kg·天）），持续60天，并同时给予丹皮酚（50、100mg/（kg·天））。结果表明，丹皮酚显著改善了D-半乳糖处理小鼠在莫里斯水迷宫试验和跳台被动回避试验中的学习和记忆能力。进一步研究表明，丹皮酚改善认知缺陷的作用与其抑制D-半乳糖处理小鼠大脑生化变化的能力有关。丹皮酚提高了D-半乳糖处理小鼠脑中乙酰胆碱（Ach）和谷胱甘肽（GSH）水平，恢复了超氧化物歧化酶（SOD）和钠钾-腺苷三磷酸酶（Na⁺，K⁺-ATPase）活性，但降低了胆碱酯酶（AchE）活性和丙二醛（MDA）水平。此外，丹皮酚改善了D-半乳糖处理小鼠海马和颞叶皮质的神经元损伤。这些结果表明，丹皮酚具有抗衰老功效，可能在神经退行性疾病的治疗中具有潜力。
