[一种草药化合物对尾部悬吊大鼠肌肉纤维肌球蛋白三磷酸腺苷酶活性及肌肉萎缩的预防作用]
[The preventive effects of one herbal compound on activities of myosin adenosine triphosphatase of muscle fibers and muscle atrophy in tail-suspended rat].
作者信息
Zhang Hai-Xiang, He Zhi-Xian, Gao Yon-Fang
机构信息
College of Life Sciences, Northwest University, Xi'an 710069, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Aug;24(3):367-72.
AIM
To study the effect of radix-astragali compound(RC) on muscle atrophy in tail-suspended rats. Muscle weight, fiber type distribution, cross-sectional area (CSA), and activity of myosin adenosine triphosphatase (ATPase) in rat soleus muscle were investigated.
METHODS
The tail-suspended rats were subjected to a 14 days simulated weightlessness, during which period, RC or saltwater was given via intragastric instillation during tail suspension. The changes of soleus muscle weight were scaled by muscle-to-body weight ratio. The activities of myosin ATPase of muscle fibers were detected by method of Ca(2+) -ATPase.
RESULTS
After a 14 days tail suspension it was found: in rats treated with RC, soleus muscle-to-body weight ratio rose by 33.33% (P < 0.01), both CSA of type I and II fiber drastically enhanced by(143.03%, P < 0.01; 83.25%, P < 0.01), the percentage of type I fiber significantly declined compared to the untreated rats.
CONCLUSION
RC is able to effectively prevent muscle atrophy caused by tail suspension and restrain the increase in the myosin ATPase activities caused by simulated weightlessness.
目的
研究黄芪复方（RC）对尾部悬吊大鼠肌肉萎缩的影响。观察大鼠比目鱼肌的肌肉重量、纤维类型分布、横截面积（CSA）及肌球蛋白腺苷三磷酸酶（ATPase）活性。
方法
对尾部悬吊大鼠进行14天模拟失重处理，在此期间，于尾部悬吊过程中经胃内灌注给予RC或盐水。比目鱼肌重量变化以肌肉与体重之比衡量。采用Ca(2+) -ATPase法检测肌纤维的肌球蛋白ATPase活性。
结果
尾部悬吊14天后发现：给予RC治疗的大鼠，比目鱼肌与体重之比升高33.33%（P < 0.01），I型和II型纤维的CSA均显著增加（分别增加143.03%，P < 0.01；83.25%，P < 0.01），I型纤维百分比与未处理大鼠相比显著下降。
结论
RC能够有效预防尾部悬吊引起的肌肉萎缩，并抑制模拟失重导致的肌球蛋白ATPase活性增加。
Zotero 插件