Timakova N V, Aleshkin G I, Titova I V, Goderdzishvili M G, Zakharenko V I, Brukhanskiĭ G V, Loginova O G, Ditiatkin S Ia, Il'iashenko B N
Biull Eksp Biol Med. 1990 Mar;109(3):299-301.
The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited and expressed the hybrid plasmid PR4::Mu cts 62 genes for antibiotic resistance and temperature sensitivity. Production of the bacteriophage Mu cts 62 particles was not registered in the bacillary transcipient cells. The plasmid RP4::Mu cts 62 genes were localized in the chromosome of Bac. cereus 682 transcipient by the blot-hybridization technique with 32P-labelled DNA of the bacteriophage Mu cts 62 and the plasmid PR4. The transcipient of Bac. cereus 682 has the donor properties and transfers the RP4::Mu cts 62 genes to recipient cells of Bac. cereus DSM 318 with the frequency of 10(-6)-10(-7). The expression and transfer of the gram-negative plasmid genes in gram-positive bacterial cells are discussed.
通过携带质粒PR4::Mu cts 62的大肠杆菌供体与蜡状芽孢杆菌GP7受体细胞进行属内交配，以10(-9)的频率获得了转导子。选择了转导子克隆蜡状芽孢杆菌682，其稳定遗传并表达了杂交质粒PR4::Mu cts 62的抗生素抗性和温度敏感性基因。在杆菌转导子细胞中未检测到噬菌体Mu cts 62颗粒的产生。通过用32P标记的噬菌体Mu cts 62和质粒PR4的DNA进行印迹杂交技术，将质粒RP4::Mu cts 62基因定位在蜡状芽孢杆菌682转导子的染色体上。蜡状芽孢杆菌682的转导子具有供体特性，并以10(-6)-10(-7)的频率将RP4::Mu cts 62基因转移到蜡状芽孢杆菌DSM 318的受体细胞中。讨论了革兰氏阴性质粒基因在革兰氏阳性细菌细胞中的表达和转移。
