[质粒RP4::Mu cts62对霍乱弧菌染色体基因的动员]
[Mobilization of Vibrio cholerae chromosomal genes by plasmid RP4::Mu cts62].
作者信息
Skavronskaia A G, Aleshkin G I, Tiganova I G, Shaginian I A
出版信息
Genetika. 1982 Feb;18(2):227-34.
The RP4::Mu cts62 plasmid was constructed in Escherichia coli cells and subsequently transferred by conjugation into the cells of Vibrio cholerae. This plasmid had been shown to mobilize chromosomal genes of V. cholerae during intrageneric matings. The frequency of mobilization is higher in matings at 37 degrees C, the temperature which is semipermissive for temperature sensitive Mu cts62 phage. This is only true for strains harbouring RP4::Mu cts62, but not for strains containing the RP4 plasmid. Frequencies of mobilization per transferred plasmid exceeded conspicuously the known frequency for the mobilizing activity of the P-factor of V. cholerae. Transconjugants selected for chromosomal markers carried no traces of RP4 plasmid or Mu cts62 markers. This feature of their use in the genetical analysis of V. cholerae. Reasons for the absence of plasmid markers in recombinants are being discussed.
RP4::Mu cts62质粒在大肠杆菌细胞中构建而成，随后通过接合转移至霍乱弧菌细胞中。该质粒已被证明在属内交配过程中能动员霍乱弧菌的染色体基因。在37摄氏度交配时，动员频率更高，此温度对半温度敏感型Mu cts62噬菌体而言是半允许温度。这仅适用于携带RP4::Mu cts62的菌株，而不适用于含有RP4质粒的菌株。每个转移质粒的动员频率显著超过了霍乱弧菌P因子已知的动员活性频率。为染色体标记选择的转接合子未携带RP4质粒或Mu cts62标记的痕迹。这是其在霍乱弧菌遗传分析中的使用特点。文中讨论了重组体中缺乏质粒标记的原因。
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