Kaplan A M, Akhverdian V Z, Krylov V N
Genetika. 1989 Aug;25(8):1384-90.
Hybrid plasmids obtained as a result of Mu phage insertions into the RP4::D3112 plasmid in Escherichia coli cells were studied. Stable maintenance of RP4::D3112 plasmid in E. coli cells was provided by using the D3112 phage genome with a point polar mutation in the A gene which prevented early genes' expression. The presence of D3112A- in the RP4 plasmid has been shown to have no effect on efficiency of phage Mu transposition into this plasmid. Moreover, RP4 and D3112 genomes were equivalent targets for Mu integration. The integration of transposable phage into genome of nonrelated phage can be used as one of the approaches to construct recombinant phage genomes in vivo in the absence of DNA homology.
对通过 Mu 噬菌体插入大肠杆菌细胞中的 RP4::D3112 质粒而获得的杂交质粒进行了研究。通过使用在 A 基因中具有点极性突变的 D3112 噬菌体基因组来确保 RP4::D3112 质粒在大肠杆菌细胞中的稳定维持，该突变可阻止早期基因的表达。已表明 RP4 质粒中 D3112A- 的存在对 Mu 噬菌体转座到该质粒的效率没有影响。此外，RP4 和 D3112 基因组是 Mu 整合的等效靶标。在不存在 DNA 同源性的情况下，可将转座噬菌体整合到不相关噬菌体的基因组中用作在体内构建重组噬菌体基因组的方法之一。
