Katoh Y, Komuro I, Takaku F, Yamaguchi H, Yazaki Y
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Circ Res. 1990 Jul;67(1):235-9. doi: 10.1161/01.res.67.1.235.
The expression of guanine nucleotide-binding protein (G-protein) genes (Gs alpha, Go alpha, Gi alpha 1, Gi alpha 2, and Gi alpha 3) was examined in the ventricle of cardiomyopathic Syrian hamsters of the Bio14.6 strain (10-35 weeks old). Northern blot analysis of total cellular RNA revealed that all G-protein genes except Gi alpha 1 were expressed in the ventricle of Syrian hamsters. Gs alpha and Gi alpha 2 genes were abundantly expressed. The expression levels of the Gs alpha and Gi alpha 2 messenger RNAs in Bio14.6 ventricles were lower than the levels in ventricles of the F1B hamster strain; the abundance of Go alpha and Gi alpha 3 messenger RNAs did not change markedly. Moreover, the messenger RNA levels of Gs alpha and Gi alpha 2 decreased as the stage of cardiomyopathy progressed. Since G-proteins are linked to adenylate cyclase, these alterations of G-protein messenger RNA levels may be related to reduced contractility of cardiomyopathic heart.
在10至35周龄的Bio14.6品系心肌病叙利亚仓鼠的心室中，检测了鸟嘌呤核苷酸结合蛋白（G蛋白）基因（Gsα、Goα、Giα1、Giα2和Giα3）的表达。对总细胞RNA进行的Northern印迹分析显示，除Giα1外，所有G蛋白基因均在叙利亚仓鼠心室中表达。Gsα和Giα2基因大量表达。Bio14.6心室中Gsα和Giα2信使RNA的表达水平低于F1B仓鼠品系心室中的水平；Goα和Giα3信使RNA的丰度没有明显变化。此外，随着心肌病阶段的进展，Gsα和Giα2的信使RNA水平降低。由于G蛋白与腺苷酸环化酶相关联，G蛋白信使RNA水平的这些改变可能与心肌病心脏收缩力降低有关。
