Morrison A D
Division of Endocrinology, Faculty of Medicine, University of British Columbia, Vancouver.
Clin Invest Med. 1990 Jun;13(3):119-22.
The relationship was examined between increased polyol pathway activity and the changes in water content, respiration, and glycolysis that occur when tubular segments of rabbit aortic smooth muscle cells are incubated with elevated glucose concentrations. The presence of 1.0 mmol/L ibuprofen resulted in a 65% reduction in fructose production by tissue incubated with 50 mmol/L glucose. This was associated with an increase in intracellular glucose and decreases in aortic smooth muscle sorbitol and fructose consistent with an inhibition of aldose reductase. Inhibition of increased polyol pathway activity usually observed in tissue incubated with 50 mmol/L glucose, is accompanied by a decrease in tissue water, an increase in oxygen uptake, and a decrease in lactate production. This suggests a causal relationship between increased polyol pathway activity and the changes in the aortic water content and metabolism induced by an elevated medium glucose concentration, although this would not be predicted by the osmotic hypothesis. The mechanism(s) responsible for the prevention of metabolic changes seen in an elevated glucose concentration by the aldose reductase inhibitor remain to be elucidated.
研究了兔主动脉平滑肌细胞管状节段在高糖浓度孵育时，多元醇途径活性增加与水分含量、呼吸作用及糖酵解变化之间的关系。1.0 mmol/L布洛芬的存在使在50 mmol/L葡萄糖中孵育的组织果糖生成量减少了65%。这与细胞内葡萄糖增加、主动脉平滑肌山梨醇和果糖减少有关，这与醛糖还原酶的抑制作用相符。通常在50 mmol/L葡萄糖中孵育的组织中观察到的多元醇途径活性增加受到抑制时，会伴随着组织水分减少、氧摄取增加以及乳酸生成减少。这表明多元醇途径活性增加与高糖培养基浓度引起的主动脉水分含量和代谢变化之间存在因果关系，尽管渗透假说无法预测这一点。醛糖还原酶抑制剂预防高糖浓度下所见代谢变化的机制仍有待阐明。
