Laboratory of Molecular Microbiology and Antimicrobials, Department of Pathology and Experimental Therapeutics, Faculty of Medicine, University Clinic of Podiatry, University of Barcelona, Spain.
Med Mycol. 2011 Jul;49(5):495-9. doi: 10.3109/13693786.2010.541949. Epub 2010 Dec 13.
Due to the increase of choices relative to antifungals, there is a need to improve the standardization of in vitro methods used to determine the antifungal susceptibility of fungal pathogens. Our study evaluated the in vitro susceptibility of filamentous fungi isolated from patients with toenail onychomycosis against itraconazole, ciclopirox, eberconazole, fluconazole and terbinafine. The minimum inhibitory concentration (MIC) of these antifungal agents was determined with 100 isolates, including dermatophytes (70 strains) and non-dermatophyte molds (30 strains). The susceptibility of fungal isolates was measured by using a technique modified for dermatophytes (0.5 × 10(3)-0.5 × 10(4) conidia/ml as inocula) which followed the procedures described by the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST) and the Clinical and Laboratory Standard Institute (CLSI M38-A). MIC ranges were 0.016-8.0 μg/ml for itraconazole, ciclopirox and eberconazole, 0.063-32.0 μg/ml for fluonazole, and 0.004-2.0 μg/ml for terbinafine. In vitro susceptibility tests indicated that eberconazole has a broad antimicrobial profile, including dermatophytes, as well as other filamentous fungi. Terbinafine was active (0.016-0.250 μg/ml) against dermatophytes.
由于可供选择的抗真菌药物增多，因此需要提高体外方法来检测真菌病原体抗真菌敏感性的标准化程度。我们的研究评估了从甲真菌病患者的趾甲中分离出的丝状真菌对伊曲康唑、环吡酮、艾伯康唑、氟康唑和特比萘芬的体外敏感性。用 100 株分离株（包括皮肤癣菌[70 株]和非皮肤癣菌霉菌[30 株]）测定这些抗真菌药物的最低抑菌浓度（MIC）。采用改良的皮肤癣菌技术（接种物为 0.5×10(3)-0.5×10(4)个孢子/ml）测量真菌分离株的敏感性，该技术遵循抗真菌药敏试验欧洲委员会抗菌药物敏感性试验委员会（AFST-EUCAST）和临床实验室标准化研究所（CLSI M38-A）描述的程序。伊曲康唑、环吡酮和艾伯康唑的 MIC 范围为 0.016-8.0 μg/ml，氟康唑为 0.063-32.0 μg/ml，特比萘芬为 0.004-2.0 μg/ml。体外药敏试验表明，艾伯康唑具有广谱抗菌谱，包括皮肤癣菌和其他丝状真菌。特比萘芬对皮肤癣菌具有活性（0.016-0.250 μg/ml）。
