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用含有编码流产布鲁氏菌31千道尔顿蛋白的重组质粒的减毒鼠伤寒沙门氏菌对小鼠进行口服免疫。

Oral immunization of mice with attenuated Salmonella typhimurium containing a recombinant plasmid which codes for production of a 31-kilodalton protein of Brucella abortus.

作者信息

Stabel T J, Mayfield J E, Tabatabai L B, Wannemuehler M J

机构信息

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames 50011.

出版信息

Infect Immun. 1990 Jul;58(7):2048-55. doi: 10.1128/iai.58.7.2048-2055.1990.

DOI:10.1128/iai.58.7.2048-2055.1990
PMID:2114357
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC258774/
Abstract

Salmonella typhimurium chi 4064, an attenuated delta cya delta crp mutant of S. typhimurium SR-11, was used as a carrier for the plasmid pBA31-R7. This plasmid codes for the expression of a 31-kilodalton (kDa) protein from Brucella abortus (BCSP31). Recombinant S. typhimurium chi 4064(pBA31-R7) expressed BCSP31 in vitro as shown by Western blot (immunoblot) analysis. The plasmid was stable in vitro and in vivo and did not affect the ability of the mutant to invade and colonize the small intestine, mesenteric lymph nodes, liver, or spleen of BALB/cByJ mice. Animals orally immunized with S. typhimurium chi 4064(pBA31-R7) developed serum and intestinal antibody responses to the B. abortus 31-kDa protein and to salmonella endotoxin as measured by enzyme-linked immunosorbent assay. Mice orally immunized with S. typhimurium chi 4064pBA31-R7 did not develop a delayed-type hypersensitivity following a footpad injection with recombinant BCSP31. Antigen-specific blastogenic data also support these in vivo results. All data indicate that this route of antigen delivery is effective for stimulating antibody-mediated immunity but that the B. abortus 31-kDa protein is a poor immunogen for inducing a cell-mediated immune response in BALB/cByJ mice.

摘要

鼠伤寒沙门氏菌chi 4064是鼠伤寒沙门氏菌SR - 11的一种减毒Δcya Δcrp突变株，被用作质粒pBA31 - R7的载体。该质粒编码流产布鲁氏菌的一种31千道尔顿（kDa）蛋白（BCSP31）的表达。如蛋白质印迹（免疫印迹）分析所示，重组鼠伤寒沙门氏菌chi 4064(pBA31 - R7)在体外表达BCSP31。该质粒在体外和体内均稳定，且不影响突变株侵袭和定殖于BALB/cByJ小鼠小肠、肠系膜淋巴结、肝脏或脾脏的能力。通过酶联免疫吸附测定法检测，用鼠伤寒沙门氏菌chi 4064(pBA31 - R7)经口免疫的动物对流产布鲁氏菌31 - kDa蛋白和沙门氏菌内毒素产生了血清和肠道抗体反应。用鼠伤寒沙门氏菌chi 4064pBA31 - R7经口免疫的小鼠在足垫注射重组BCSP31后未出现迟发型超敏反应。抗原特异性增殖数据也支持这些体内实验结果。所有数据表明，这种抗原递送途径对于刺激抗体介导的免疫是有效的，但流产布鲁氏菌31 - kDa蛋白在BALB/cByJ小鼠中是一种诱导细胞介导免疫反应的弱免疫原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/86f6c241f06b/iai00055-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/6d9d7958153b/iai00055-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/f37a0b12136f/iai00055-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/af28a2259233/iai00055-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/86f6c241f06b/iai00055-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/6d9d7958153b/iai00055-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/f37a0b12136f/iai00055-0037-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/af28a2259233/iai00055-0037-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e72/258774/86f6c241f06b/iai00055-0039-a.jpg

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