作为食品级疫苗的[具体生物]中基因的克隆与表达
Cloning and Expression of Gene in as a Food Grade Vaccine.
作者信息
Azizpour Maghvan Maryam, Jafari Parvaneh, Hoseini Seyed Davood, Behrozikhah Ali Mohammad
机构信息
Department of Microbiology, Islamic Azad University, Arak Branch, Arak, Iran.
Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization, Arak, Iran.
出版信息
Avicenna J Med Biotechnol. 2019 Jul-Sep;11(3):264-267.
BACKGROUND
Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. gene is a good candidate for brucellosis vaccine and investigations showed that ( with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive.
METHODS
gene was cloned in food grade pNZ 8149 vector and expressed in NZ 3900.
RESULTS
Results showed that we can produce a food-grade recombinant producing the BP26 protein.
CONCLUSION
In this study, for Future evaluation about ability of as a live delivery vector, a food-grade recombinant producing the BP26 protein was produced.
背景
布鲁氏菌病在发展中国家仍是一个重要的健康问题，目前正在进行寻找有效疫苗的研究。基因是布鲁氏菌病疫苗的一个良好候选者，研究表明（具有几个积极特性，作为活载体用于蛋白质表达很有吸引力。这些快速生长的细菌不需要通气，易于处理，没有外毒素、内毒素和蛋白酶，因此培养成本低廉。
方法
将基因克隆到食品级pNZ 8149载体中，并在NZ 3900中表达。
结果
结果表明，我们可以生产出产生BP26蛋白的食品级重组体。
结论
在本研究中，为了未来评估作为活载体的能力，生产出了产生BP26蛋白的食品级重组体。
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