Kotowski Michal, Niedzielski Artur, Niedzielska Grazyna, Lachowska-Kotowska Patrycja
Department of Pediatric Otolaryngology, Phoniatrics and Audiology, Medical University of Lublin, Poland.
Int J Pediatr Otorhinolaryngol. 2011 Feb;75(2):265-9. doi: 10.1016/j.ijporl.2010.11.014. Epub 2010 Dec 8.
To assess dendritic cells and lymphocyte subpopulations of adenoid and peripheral blood in patients with adenoid hypertrophy and otitis media with effusion (OME). To explain the influence of immunological status of adenoid on the development of OME.
The examined group covered 123 surgically treated patients due to adenoid hypertrophy (39 children with coexisting OME). Preoperative clinical examination included anamnesis and otorhinolaryngological examination with nasofiberoscopy, videootoscopy and assessment of the hearing organ. The material of the study were surgically removed adenoids and peripheral blood samples prepared and then analyzed by the means of tri-colour flow cytometry. The obtained results were statistically analyzed.
Significant differences between patients with adenoid hypertrophy with coexisting OME and children without OME concerning CD19+CD69+, CD3+CD69+, CD4+CD69+CD8+CD69+ and CD19+CD25+ cells were observed. No statistical differences were revealed in the blood of patients with and without OME. Several statistical differences were noted between the adenoid and peripheral blood in patients with otitis media with effusion concerning BDCA-2+/CD123+ cells, CD3+ and CD19+ lymphocytes with the markers of their activation.
The adenoid is involved in local immune response regardless of constitutional immunological mechanisms in patients with OME coexisting with adenoid hypertrophy. Significantly lower percentage of CD3+CD69+, CD4+CD69+, CD8+CD69+ and CD19+CD69+ cells of adenoid in patients with OME attests to reduced T-cells activation of the adenoid in relation to patients without OME.
评估腺样体肥大合并中耳积液（OME）患者腺样体及外周血中的树突状细胞和淋巴细胞亚群。解释腺样体免疫状态对OME发生发展的影响。
研究组包括123例因腺样体肥大接受手术治疗的患者（39例合并OME的儿童）。术前临床检查包括病史采集以及通过鼻纤维镜、耳内镜检查和听力器官评估进行的耳鼻咽喉科检查。研究材料为手术切除的腺样体和制备的外周血样本，然后通过三色流式细胞术进行分析。对所得结果进行统计学分析。
观察到腺样体肥大合并OME的患者与未合并OME的儿童在CD19+CD69+、CD3+CD69+、CD4+CD69+、CD8+CD69+和CD19+CD25+细胞方面存在显著差异。在有或无OME的患者血液中未发现统计学差异。在中耳积液患者的腺样体和外周血之间，观察到BDCA-2+/CD123+细胞、CD3+和CD19+淋巴细胞及其活化标志物存在若干统计学差异。
在腺样体肥大合并OME的患者中，无论全身免疫机制如何，腺样体均参与局部免疫反应。OME患者腺样体中CD3+CD69+、CD4+CD69+、CD8+CD69+和CD19+CD69+细胞的百分比显著降低，证明与未合并OME的患者相比，腺样体中T细胞活化减少。
