Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str. 16/10, 117997 Moscow, Russian Federation.
Plant Physiol Biochem. 2011 Feb;49(2):131-7. doi: 10.1016/j.plaphy.2010.10.008. Epub 2010 Nov 18.
From seeds of Nigella sativa L. (Ranunculaceae), an endemic plant of Uzbekistan, two novel defensins named Ns-D1 and Ns-D2, were isolated and sequenced. The peptides differ by a single amino acid residue and show high sequence similarity to Raphanus sativus L. defensins Rs-AFP1 and Rs-AFP2. The Ns-D1 and Ns-D2 defensins display strong although divergent antifungal activity towards a number of phytopathogenic fungi. High antifungal activity of N. sativa defensins makes them promising candidates for engineering pathogen-resistant plants.
从原产于乌兹别克斯坦的植物黑种草（毛茛科）的种子中，分离并测序得到了两种新型防御素，命名为 Ns-D1 和 Ns-D2。这两种肽仅在一个氨基酸残基上有所不同，与萝卜防御素 Rs-AFP1 和 Rs-AFP2 具有高度的序列相似性。Ns-D1 和 Ns-D2 防御素对多种植物病原菌具有较强的抗真菌活性，尽管活性有所不同。黑种草防御素的高抗真菌活性使它们成为工程抗病植物的有前途的候选物。
