Univ Paris-Sud, UMR CNRS 8612, Faculté de Pharmacie, 5 rue Jean-Baptiste Clément, 92296 Châtenay-Malabry Cedex, France.
J Colloid Interface Sci. 2011 Feb 15;354(2):467-77. doi: 10.1016/j.jcis.2010.11.019. Epub 2010 Dec 8.
Natural biopolymer stabilized oil-in-water emulsions were formulated using β-lactoglobulin (β-lg), gum arabic (GA), and β-lg:GA solutions as an alternative to synthetic surfactants. Emulsions using these biopolymers and their complexes were formulated varying the biopolymer total concentration, the protein-to-polysaccharide ratio, and the emulsification protocol. This work showed that whereas β-lg enabled the formulation of emulsions at concentration as low as 0.5 (w/w)%, GA allowed to obtain emulsions at concentrations equal to or higher than 2.5 (w/w)%. In order to improve emulsion stability, β-lg and GA were complexed through strong attractive electrostatic interactions. GA solution had to be added to previously prepared β-lg emulsions in order to obtain stable emulsions. Interfacial tension and interfacial rheological measurements allowed a better understanding of the possible stabilizing mechanism. β-lg and GA both induced a very effective decrease in interfacial tension and showed interfacial elastic behaviour. In the mixed system, β-lg adsorbed at the interface and GA electrostatically bound to it, leading to the formation of a bi-layer stabilized emulsion. However, emulsion stability was not improved compared to β-lg stabilized emulsion, probably due to depletion or bridging flocculation.
采用β-乳球蛋白（β-lg）、阿拉伯胶（GA）和β-lg:GA 溶液作为替代合成表面活性剂，制备了天然生物聚合物稳定的水包油乳液。通过改变生物聚合物总浓度、蛋白质与多糖的比例以及乳化方案，制备了使用这些生物聚合物及其复合物的乳液。研究表明，虽然β-lg 能够在低至 0.5（w/w）%的浓度下制备乳液，但 GA 允许获得浓度等于或高于 2.5（w/w）%的乳液。为了提高乳液稳定性，通过强静电吸引力将β-lg 和 GA 进行复合。为了获得稳定的乳液，必须将 GA 溶液添加到先前制备的β-lg 乳液中。界面张力和界面流变学测量有助于更好地理解可能的稳定机制。β-lg 和 GA 都能非常有效地降低界面张力，并表现出界面弹性行为。在混合体系中，β-lg 吸附在界面上，GA 则通过静电作用与之结合，形成双层稳定乳液。然而，与β-lg 稳定的乳液相比，乳液稳定性并没有得到改善，这可能是由于耗尽或桥接絮凝所致。
