Department of General Surgery, Ninth People's Hospital affiliated to Shanghai Jiaotong University, School of Medicine, Shanghai 200011, China.
Int J Biol Macromol. 2011 Apr 1;48(3):386-91. doi: 10.1016/j.ijbiomac.2010.12.005. Epub 2010 Dec 8.
A water-soluble polysaccharide was extracted from the fruits of Chinese jujube by boiled water extraction and ethanol precipitation. Monosaccharide composition and the structure of the polysaccharide were determined by gas spectroscopy and Fourier transform IR (FT-IR) spectroscopy. The components were glucose (23%), xylose (31.3%), mannose (12.9%) and fructose (21.6%). Six distinct-absorbance peaks, at 3290.20, 2928.66, 1616.20, 1342.96, 1254.35cm(-1), 1025.43, 917, 28 and 816.57cm(-1) in the infrared (IR) spectra of Chinese jujube polysaccharides were the characteristic of polysaccharide compound. Chinese jujube polysaccharides have effective reducing power, free radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, and metal chelating activities. This antioxidant property depends on concentration and increases with increased amount of sample. In addition, ischemia/reperfusion could stimulate oxidative injury in rabbits' intestine. Chinese jujube polysaccharides could still effectively reduce intestine MDA level and increase antioxidant enzyme activities in rabbits with ischemia/reperfusion (I/R) of the small intestine.
从大枣果实中用水提取多糖，用沸水提取和乙醇沉淀。通过气相光谱和傅里叶变换红外（FT-IR）光谱法测定多糖的单糖组成和结构。成分是葡萄糖（23%），木糖（31.3%），甘露糖（12.9%）和果糖（21.6%）。大枣多糖在 3290.20、2928.66、1616.20、1342.96、1254.35cm(-1)、1025.43、917、28 和 816.57cm(-1)处有六个明显的吸收峰，这些是多糖化合物的特征。大枣多糖具有有效的还原能力、自由基清除能力、超氧阴离子自由基清除能力、过氧化氢清除能力和金属螯合能力。这种抗氧化性能取决于浓度，并随样品量的增加而增加。此外，缺血/再灌注可刺激兔肠的氧化损伤。大枣多糖仍能有效降低缺血/再灌注（I/R）兔小肠 MDA 水平，增加抗氧化酶活性。
