Wasylyk B, Wasylyk C, Flores P, Begue A, Leprince D, Stehelin D
Laboratoire de Génétique Moléculaire des Eucaryotés du CNRS, Strasbourg, France.
Nature. 1990 Jul 12;346(6280):191-3. doi: 10.1038/346191a0.
Cell transformation by oncogenes leads to changes in gene expression. A key event in this process seems to be activation of the transcription factors AP-1 and PEA 3. Their synergistic activities are required for efficient activation of transcription from different promoters by many different oncogenes, serum growth factors and the tumour promoter TPA. We show here that the products of the ets-1 and -2 proto-oncogenes, whose biological function was previously unknown, are transcription factors that activate transcription through the PEA 3 motif. The p68c-ets-1 protein specifically binds to DNA and contains a transcriptional activation domain. The ets-like gene family therefore seems to encode a new family of transcription factors, apparently unrelated to other transcription factors. The p68c-ets-1 protein cooperates with c-Fos and c-Jun (components of AP-1) for activation of transcription from the oncogene-responsive domain of the polyoma enhancer, indicating that combined activity of all three oncoproteins could be involved in the response of cells to growth stimuli.
癌基因导致的细胞转化会引起基因表达的变化。这一过程中的一个关键事件似乎是转录因子AP-1和PEA 3的激活。许多不同的癌基因、血清生长因子和肿瘤启动子TPA要高效激活不同启动子的转录，都需要它们的协同活性。我们在此表明，ets-1和-2原癌基因的产物，其生物学功能此前未知，是通过PEA 3基序激活转录的转录因子。p68c-ets-1蛋白特异性结合DNA并含有一个转录激活结构域。因此，ets样基因家族似乎编码了一个新的转录因子家族，显然与其他转录因子无关。p68c-ets-1蛋白与c-Fos和c-Jun（AP-1的组成成分）协同作用，以激活来自多瘤病毒增强子的癌基因反应域的转录，这表明所有三种癌蛋白的联合活性可能参与细胞对生长刺激的反应。
