School of Veterinary Sciences, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom.
Vet Microbiol. 2011 May 5;149(3-4):478-81. doi: 10.1016/j.vetmic.2010.11.016. Epub 2010 Nov 19.
In order to confirm a microscopic diagnosis of 'eperythrozoonosis' made over 40 years ago in a captive owl monkey (Aotus trivirgatus), DNA was extracted from archived fixed and stained blood smears and subjected to generic haemotropic mycoplasma (haemoplasma) quantitative real-time PCR (qPCR) and a human glyceraldehyde-3-phosphate dehydrogenase qPCR as an amplification control. The qPCRs confirmed the extraction of host DNA from the samples and the presence of a haemoplasma species. Partial 16S rRNA and ribonuclease P ribosomal gene fragments were amplified by PCR, cloned and sequenced. Sequence data and phylogeny showed the owl monkey haemoplasma to lie in the haemominutum clade of haemoplasmas, most closely related to 'Candidatus Mycoplasma kahaneii'. This study confirms the use of generic haemoplasma qPCRs to successfully amplify haemoplasma DNA from fixed, stained and archived blood smears from the early 1970s and provides molecular confirmation of the existence of a novel haemoplasma species in an owl monkey, for which the name 'Candidatus Mycoplasma aoti' sp. nov. is proposed.
为了确认 40 多年前在笼养的卷尾猴（Aotus trivirgatus）中做出的“边缘无浆体病”的微观诊断，从存档的固定和染色血涂片提取 DNA，并进行通用血质支原体（血质支原体）定量实时 PCR（qPCR）和人类甘油醛-3-磷酸脱氢酶 qPCR 作为扩增对照。qPCR 证实从样本中提取了宿主 DNA，并存在血质体物种。通过 PCR 扩增、克隆和测序，扩增了部分 16S rRNA 和核糖核酸酶 P 核糖体基因片段。序列数据和系统发育表明，猫头鹰猴血质体位于血质体的血细足菌群中，与“候选支原体 kahaneii”最为密切相关。本研究证实，通用血质体 qPCR 可成功从 20 世纪 70 年代早期的固定、染色和存档血涂片扩增血质体 DNA，并为在猫头鹰猴中存在一种新型血质体提供了分子确认，为此提议了一种新的血质体物种，命名为“候选支原体 aoti”sp. nov.。
