Sashida Hinako, Suzuki Yoshihisa, Rokuhara Sou, Nagai Kazuya, Harasawa Ryô
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan.
J Vet Med Sci. 2014 Jan;76(1):97-101. doi: 10.1292/jvms.13-0332. Epub 2013 Aug 26.
The prevalence of hemotropic mycoplasmas in wild monkeys is largely unknown. Here, we report the presence of hemoplasmas in blood specimens collected from wild Japanese monkeys (Macaca fuscata) tentatively captured for ecological survey in Mie prefecture, Japan. We examined 9 monkeys using hemoplasma-specific real-time PCR and found all of them positive for a hemoplasma infection. The 16S rRNA gene and 16S to 23S rRNA intergenic spacer region of the hemoplasma detected in wild monkeys were amplified using end-point PCR. The nucleotide sequences of the PCR products were further determined and compared to those of other hemoplasmas. Our examinations revealed a wide prevalence of a hemoplasma strain in Japanese monkeys, which was similar to 'Candidatus Mycoplasma haemomacaque' reported in cynomolgus monkeys (Macaca fascicularis). Pathogenic traits of this hemoplasma strain remain unexplored.
野生猴子中血源性支原体的流行情况在很大程度上尚不清楚。在此，我们报告在从日本三重县为生态调查而临时捕获的野生日本猕猴（Macaca fuscata）采集的血液样本中存在血支原体。我们使用血支原体特异性实时PCR检测了9只猴子，发现它们均感染了血支原体呈阳性。通过终点PCR扩增野生猴子中检测到的血支原体的16S rRNA基因和16S至23S rRNA基因间隔区。进一步测定PCR产物的核苷酸序列并与其他血支原体的序列进行比较。我们的检测显示日本猕猴中一种血支原体菌株广泛流行，这与食蟹猴（Macaca fascicularis）中报道的“嗜猴血支原体（Candidatus Mycoplasma haemomacaque）”相似。这种血支原体菌株的致病特性仍未得到探索。
