Zhuang Q J, Zhang H J, Lin R Q, Sun M F, Liang X J, Qin X W, Pu W J, Zhu X Q
College of Veterinary Medicine, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou, Guangdong Province 510642, People's Republic of China.
Trop Anim Health Prod. 2009 Apr;41(4):689-92. doi: 10.1007/s11250-008-9242-2. Epub 2008 Oct 24.
In the present study, polymerase chain reaction (PCR) assay was used to detect haemoplasmas (haemotropic bacteria) in 40 clinically healthy pet dogs in Foshan city, Guangdong Province, China, and one dog was found positive. Comparison of its 16S ribosomal DNA (rDNA) sequence with relevant sequences showed that the isolated haemoplasma had greater sequence identity to feline species "Candidatus Mycoplasma haemominutum" (99%) than to "Candidatus Mycoplasma haematoparvum" (95%). This result, for the first time, indicates the presence of the feline "Candidatus Mycoplasma haemominutum" in Chinese dogs and it represents the first survey of its kind in China by using PCR assay. The results indicated that dog may represent one of the hosts for the feline "Candidatus Mycoplasma haemominutum".
在本研究中，采用聚合酶链反应（PCR）检测法对中国广东省佛山市40只临床健康的宠物犬进行血支原体（血源性细菌）检测，发现1只犬呈阳性。将其16S核糖体DNA（rDNA）序列与相关序列进行比较，结果显示，分离出的血支原体与猫源“溶血微小支原体（暂定种）”的序列一致性更高（99%），而与“血细小支原体（暂定种）”的一致性为95%。该结果首次表明中国犬体内存在猫源“溶血微小支原体（暂定种）”，且这是中国首次采用PCR检测法进行此类调查。结果表明，犬可能是猫源“溶血微小支原体（暂定种）”的宿主之一。
