Golyshevskaia V I, Makarevich N M
Probl Tuberk. 1990(3):41-4.
Soviet chlorhexedin bigluconicum (CHBG) was used for sputum treatment. 129 sputum specimens were investigated. Among them 45 specimens were bacterioscopically negative. The rest contained low, moderate and high numbers of tubercle bacilli. The sputum was incubated on the Löwenstein-Jensen and Finn-II media. Comparison of two treatment methods (with Na3PO4 and CHBG) showed that CHBG had a more sparing effect on tubercle bacilli. The most marked effect was observed with incubation of oligobacillar materials and materials containing low numbers of tubercle bacilli. The highest differences were revealed when the materials were incubated on the Löwenstein-Jensen medium. It was shown that isolation of the cultures from oligobacillar materials increased by 4-14.3 per cent. The growth intensity and rate were higher. The majority of the cultures grew in 3-4 weeks whereas with using Na3PO4 part of the cultures grew during the period between the 6th and the 8th weeks. When the number of tubercle bacilli was high irrespective of the treatment procedure the number of the grown cultures was the same. The germination level was low and amounted to 3.9 per cent after treatment with Na3PO4 and to 2.3 per cent after treatment with CHBG. The method using CHBG is simple, economic and valuable in laboratory practice.
苏联的葡萄糖酸氯己定(CHBG)被用于痰液治疗。对129份痰液标本进行了研究。其中45份标本细菌学检查为阴性。其余标本含有少量、中等数量和大量结核杆菌。痰液在罗-琴培养基和芬兰-II培养基上培养。两种治疗方法(使用磷酸钠和CHBG)的比较表明,CHBG对结核杆菌的作用更温和。在培养含少量结核杆菌的材料和含少量结核杆菌的材料时观察到最明显的效果。当材料在罗-琴培养基上培养时,差异最为显著。结果表明,从含少量结核杆菌的材料中分离培养物的比例提高了4%-14.3%。生长强度和速度更高。大多数培养物在3-4周内生长,而使用磷酸钠时,部分培养物在第6至8周期间生长。当结核杆菌数量较多时,无论治疗方法如何,生长的培养物数量相同。发芽水平较低,用磷酸钠治疗后为3.9%,用CHBG治疗后为2.3%。使用CHBG的方法简单、经济,在实验室实践中有价值。