Department of Animal Molecular Physiology and Laboratory of Cellular Neurobiology, Institute of Zoology, Wrocław University, Cybulskiego 30, 50-205 Wrocław, Poland.
Biochem Biophys Res Commun. 2011 Jan 14;404(2):652-5. doi: 10.1016/j.bbrc.2010.12.033. Epub 2010 Dec 10.
Astrocytic glycogen metabolism sustains neuronal activity but its impact on basal glutamatergic synaptic transmission is not clear. To address this issue, we have compared the effect of glycogen breakdown inhibition on miniature excitatory postsynaptic currents (mEPSCs) in rat hippocampal pure neuronal culture (PNC) and in astrocyte-neuronal co-cultures (ANCC). Amplitudes of mEPSC in ANCC were nearly twice as large as in PNC with no difference in current kinetics. Inhibition of glycogen phosphorylase reduced mEPSC amplitude by roughly 40% in ANCC being ineffective in PNC. Altogether, these data indicate that astrocyte-neuronal interaction enhances basal mEPSCs in ANCC mainly due to astrocytic glycogen metabolism.
星形胶质细胞糖原代谢维持神经元活动，但它对基础谷氨酸能突触传递的影响尚不清楚。为了解决这个问题，我们比较了糖原分解抑制对大鼠海马纯神经元培养（PNC）和星形胶质细胞-神经元共培养（ANCC）中微小兴奋性突触后电流（mEPSC）的影响。ANCC 中的 mEPSC 幅度几乎是 PNC 的两倍，而电流动力学没有差异。糖原磷酸化酶的抑制作用使 ANCC 中的 mEPSC 幅度降低了约 40%，而在 PNC 中则无效。总的来说，这些数据表明，星形胶质细胞-神经元相互作用主要通过星形胶质细胞糖原代谢增强 ANCC 中的基础 mEPSC。
