幼牛胸腺中的神经氨酸酶：通过连续荧光测定法进行测定和表征。
Neuraminidase in juvenile calf thymus: determination and characterization by a continuous fluorometric assay procedure.
作者信息
Bieringer K, Zoch E
机构信息
Fachbereich 3, Theoretische Medizin, Universität des Saarlandes, Homburg/Saar, FRG.
出版信息
Thymus. 1990 Jun;15(4):233-40.
A continuous fluorometric neuraminidase assay has been developed. Within the pH range optimal for neuraminidases (from 3 to 6) the fluorescence intensity of 4-methylumbelliferone exceeds that of the glycoside 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid about 50 times (at lambda Ex = 335 nm and lambda Em = 445 nm), allowing the precise, simple and time-saving continuous fluorometric registration of enzymatically released 4-methylumbelliferone. In juvenile calf thymus a neuraminidase consisting of two components differing in pH optima and resistence to freezing and to detergents could be found. A beta-galactosidase activity in juvenile calf thymus could be proved by the same assay procedure using the synthetic substrate 4-methylumbelliferyl-beta-D-galactopyranoside.
已开发出一种连续荧光神经氨酸酶测定法。在神经氨酸酶的最佳pH范围内（3至6），4-甲基伞形酮的荧光强度比糖苷4-甲基伞形酮基-α-D-N-乙酰神经氨酸的荧光强度高约50倍（在激发波长λEx = 335 nm和发射波长λEm = 445 nm时），从而能够对酶促释放的4-甲基伞形酮进行精确、简单且省时的连续荧光记录。在幼年小牛胸腺中，可以发现一种由两种成分组成的神经氨酸酶，这两种成分在最适pH、抗冻性和对去污剂的耐受性方面存在差异。使用合成底物4-甲基伞形酮基-β-D-吡喃半乳糖苷，通过相同的测定程序可以证明幼年小牛胸腺中存在β-半乳糖苷酶活性。
Zotero 插件