Cha Ra Mi, Khatri Mahesh, Mutnal Manohar, Sharma Jagdev M
Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN 55108, USA.
Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):30-6. doi: 10.1016/j.vetimm.2010.11.006. Epub 2010 Nov 19.
Commercial turkey eggs, free of antibodies to avian metapneumovirus subtype C (aMPV/C), were inoculated with aMPV/C at embryonation day (ED) 24. There was no detectable effect of virus inoculation on the hatchability of eggs. At 4 days post inoculation (DPI) (the day of hatch (ED 28)) and 9 DPI (5 days after hatch), virus replication was detected by quantitative RT-PCR in the turbinate, trachea and lung but not in the thymus or spleen. Mild histological lesions characterized by lymphoid cell infiltration were evident in the turbinate mucosa. Virus exposure inhibited the mitogenic response of splenocytes and thymocytes and upregulated gene expression of IFN-γ and IL-10 in the turbinate tissue. Turkeys hatching from virus-exposed eggs had aMPV/C-specific IgG in the serum and the lachrymal fluid. At 3 week of age, in ovo immunized turkeys were protected against a challenge with pathogenic aMPV/C.
将不含禽偏肺病毒C亚型（aMPV/C）抗体的商用火鸡蛋在胚胎发育第24天接种aMPV/C。病毒接种对鸡蛋孵化率未产生可检测到的影响。接种后4天（接种后天数，DPI）（孵化日（胚胎发育第28天））和接种后9天（孵化后5天），通过定量逆转录聚合酶链反应（RT-PCR）在鼻甲、气管和肺中检测到病毒复制，但在胸腺或脾脏中未检测到。鼻甲黏膜出现以淋巴细胞浸润为特征的轻度组织学病变。病毒暴露抑制了脾细胞和胸腺细胞的促有丝分裂反应，并上调了鼻甲组织中干扰素-γ（IFN-γ）和白细胞介素-10（IL-10）的基因表达。从暴露于病毒的蛋中孵化出的火鸡血清和泪液中有aMPV/C特异性免疫球蛋白G（IgG）。在3周龄时，卵内免疫的火鸡受到保护，免受致病性aMPV/C的攻击。
