Department of Chemistry, Yazd University, P.O. Box 89195-741, Yazd, Iran.
Biosens Bioelectron. 2011 Jan 15;26(5):2638-44. doi: 10.1016/j.bios.2010.11.026. Epub 2010 Nov 24.
For the detection of DNA hybridization, a new electrochemical biosensor was developed on the basis of the interaction of hematoxylin with 20-mer deoxyoligonucleotides (from human papilloma virus, HPV). The study was performed based on the interaction of hematoxylin with an alkanethiol DNA probe self-assembled gold electrode (ss-DNA/AuE) and its hybridization form (ds-DNA/AuE). The optimum conditions were found for the immobilization of HPV probe on the gold electrode (AuE) surface and its hybridization with the target DNA. Electrochemical detection of the self-assembled DNA and the hybridization process were performed by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) over the potential range where the accumulated hematoxylin at the modified electrode was electroactive. Observing a remarkable difference between the voltammetric signals of the hematoxylin obtained from different hybridization samples (non-complementary, mismatch and complementary DNAs), we confirmed the potential of the developed biosensor in detecting and discriminating the target complementary DNA from non-complementary and mismatch oligonucleotides. Under optimum conditions, the electrochemical signal had a linear relationship with the concentration of the target DNA ranging from 12.5 nM to 350.0 nM, and the detection limit was 3.8 nM.
为了检测 DNA 杂交，我们在血晶素与 20 个碱基脱氧寡核苷酸（来自人乳头瘤病毒，HPV）相互作用的基础上，开发了一种新的电化学生物传感器。该研究基于血晶素与烷硫醇 DNA 探针自组装金电极（ss-DNA/AuE）及其杂交形式（ds-DNA/AuE）的相互作用。我们找到了在金电极（AuE）表面固定 HPV 探针及其与靶 DNA 杂交的最佳条件。通过循环伏安法（CV）和差分脉冲伏安法（DPV），在修饰电极上积累的血晶素具有电化学活性的电位范围内，对自组装 DNA 和杂交过程进行了电化学检测。通过观察不同杂交样品（非互补、错配和互补 DNA）获得的血晶素的伏安信号之间存在显著差异，我们证实了所开发的生物传感器在检测和区分靶互补 DNA 与非互补和错配寡核苷酸方面的潜力。在最佳条件下，电化学信号与靶 DNA 的浓度呈线性关系，范围从 12.5 nM 到 350.0 nM，检测限为 3.8 nM。
