College of Environmental Science and Engineering, Hunan University, Changsha, PR China.
Bioresour Technol. 2011 Feb;102(3):2950-6. doi: 10.1016/j.biortech.2010.11.089. Epub 2010 Nov 25.
The goal of this study was to identify and prioritize some of the physico-chemical parameters that contributed to bacterial and fungal community compositions during agricultural waste composting. Relationships between those parameters and microbial community compositions determined by PCR-DGGE were simultaneously evaluated by redundancy analysis (RDA). The results showed that the temporal variation of bacterial community composition was significantly related to water soluble carbon (WSC), ammonium and nitrate (P<0.05), while the most variation in distribution of fungal community composition was statistically explained by pile temperature, WSC, and moisture content (P<0.05). Significant amounts of the variation (54.9% and 56.0% for bacterial and fungal species data, respectively) were explained by those parameters, suggesting that those parameters were the most likely ones to influence, or be influenced by the bacterial and fungal species. Variation partitioning analyses indicated that WSC and pile temperature showed predominant effect on the bacterial and fungal community composition, respectively.
本研究旨在确定并优先考虑一些物理化学参数，这些参数在农业废弃物堆肥过程中对细菌和真菌群落组成有贡献。通过冗余分析（RDA）同时评估这些参数与 PCR-DGGE 确定的微生物群落组成之间的关系。结果表明，细菌群落组成的时间变化与水溶性碳（WSC）、铵和硝酸盐显著相关（P<0.05），而真菌群落组成分布的最大变化在统计学上由堆温、WSC 和水分含量解释（P<0.05）。这些参数解释了大量的变化（细菌和真菌物种数据分别为 54.9%和 56.0%），表明这些参数最有可能影响或受细菌和真菌物种的影响。变分分析表明，WSC 和堆温分别对细菌和真菌群落组成有主要影响。
