Institute for Multidisciplinary Research, Kneza Višeslava 1, 11000 Beograd, Serbia.
Analyst. 2011 Jun 7;136(11):2391-6. doi: 10.1039/c0an00802h. Epub 2011 Apr 14.
There has been a lack of quick, simple and reliable methods for determination of nanoparticle size. An investigation of the size of hydrophobic (CdSe) and hydrophilic (CdSe/ZnS) quantum dots was performed by using the maximum position of the corresponding fluorescence spectrum. It has been found that fluorescence spectroscopy is a simple and reliable methodology to estimate the size of both quantum dot types. For a given solution, the homogeneity of the size of quantum dots is correlated to the relationship between the fluorescence maximum position (FMP) and the quantum dot size. This methodology can be extended to the other fluorescent nanoparticles. The employment of evolving factor analysis and multivariate curve resolution-alternating least squares for decomposition of the series of quantum dots fluorescence spectra recorded by a specific measuring procedure reveals the number of quantum dot fractions having different diameters. The size of the quantum dots in a particular group is defined by the FMP of the corresponding component in the decomposed spectrum. These results show that a combination of the fluorescence and appropriate statistical method for decomposition of the emission spectra of nanoparticles may be a quick and trusted method for the screening of the inhomogeneity of their solution.
目前，缺乏快速、简单且可靠的纳米颗粒尺寸测定方法。本研究使用相应荧光光谱的最大位置对疏水性（CdSe）和亲水性（CdSe/ZnS）量子点的尺寸进行了研究。研究发现，荧光光谱法是一种简单可靠的方法，可以用来估计这两种类型的量子点的尺寸。对于给定的溶液，量子点尺寸的均一性与荧光光谱最大位置（FMP）和量子点尺寸之间存在相关性。这种方法可以扩展到其他荧光纳米粒子。采用进化因子分析和多元曲线分辨交替最小二乘法对通过特定测量程序记录的一系列量子点荧光光谱进行分解，可以揭示具有不同直径的量子点分数的数量。特定组中的量子点的尺寸由分解谱中相应组分的 FMP 定义。这些结果表明，将荧光与适当的统计方法相结合，对纳米粒子的发射光谱进行分解，可能是筛选其溶液不均匀性的一种快速且可靠的方法。
