Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Incheon 402-751, South Korea.
Talanta. 2011 Jan 15;83(3):974-9. doi: 10.1016/j.talanta.2010.11.006. Epub 2010 Nov 9.
Three anion-exchangeable, silica-confined ionic liquids were synthesized for solid phase extraction of lactic acid from fermentation broth, followed by high-performance liquid chromatography coupled to ultraviolet detection. By comparing the adsorption isotherms of lactic acid on different silica-confined ionic liquids, interactions between the lactic acid and sorbents were investigated. The adsorbed amounts were then fitted into different adsorption isotherm equations; finally, the Langmuir equation was selected. Then the imidazolium silica with the highest adsorption capacity of lactic acid was packed into a cartridge for solid phase extraction. The loading volume of the cartridge was optimized by the Langmuir equation and geometry. After washing with distilled water and eluting with 0.25 mol L(-1) of an HCl solution, the lactic acid was separated from interference with a recovery yield of 91.9%. Furthermore, this kind of anion-exchangeable material exhibited potential for industrial applications and separation of other anionic bioactive compounds.
合成了 3 种阴离子交换型硅基离子液体，用于从发酵液中固相萃取乳酸，然后采用高效液相色谱-紫外检测法进行分析。通过比较不同硅基离子液体对乳酸的吸附等温线，考察了乳酸与吸附剂之间的相互作用。然后将吸附量拟合到不同的吸附等温线方程中，最终选择了朗缪尔方程。具有最高乳酸吸附容量的离子液体被填充到萃取柱中进行固相萃取。通过朗缪尔方程和几何原理优化了萃取柱的加载体积。用蒸馏水洗涤后，用 0.25 mol/L 的 HCl 溶液洗脱，乳酸的回收率为 91.9%，与干扰物质分离。此外，这种阴离子交换型材料具有工业应用和分离其他阴离子生物活性化合物的潜力。
