Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Talanta. 2011 Jan 15;83(3):988-93. doi: 10.1016/j.talanta.2010.11.004. Epub 2010 Nov 10.
Despite the recent, successful efforts to detect mycotoxins, new methods are still required to achieve higher sensitivity, more simplicity, higher speed, and higher accuracy at lower costs. This paper describes the determination of ochratoxin A (OTA) using corona discharge ion mobility spectrometry (IMS) in the licorice root. A quick screening and measuring method is proposed to be employed after cleaning up the extracted OTA by immunoaffinity columns. The ion mobility spectrometer is used in the inverse mode to better differentiate the OTA peak from the neighboring ones. After optimization of the experimental conditions such as corona voltage, injection port temperature, and IMS cell temperature, a limit of detection (LOD) of 0.010 ng is obtained. Furthermore, the calibration curve is found to be in the range of 0.01-1 ng with a correlation coefficient (R(2)) of 0.988. Licorice roots were analyzed for their OTA content to demonstrate the capability of the proposed method in the quantitative detection of OTA in real samples.
尽管最近在检测真菌毒素方面取得了成功，但仍需要新的方法来实现更高的灵敏度、更简单、更高的速度和更高的准确性，同时降低成本。本文描述了使用电晕放电离子淌度谱（IMS）在甘草根中检测赭曲霉毒素 A（OTA）的方法。提出了一种快速筛选和测量方法，即在免疫亲和柱提取 OTA 后进行。离子淌度谱仪采用反模式，以更好地区分 OTA 峰和相邻峰。在优化实验条件（如电晕电压、进样口温度和 IMS 池温度）后，检测限（LOD）达到 0.010 ng。此外，校准曲线的范围为 0.01-1 ng，相关系数（R(2)）为 0.988。对甘草根进行了 OTA 含量分析，以证明该方法在实际样品中定量检测 OTA 的能力。
