Department of Pharmaceutical Biochemistry, Poznań University of Medical Sciences, Poznań, Poland.
Oral Oncol. 2011 Feb;47(2):104-7. doi: 10.1016/j.oraloncology.2010.11.006. Epub 2010 Dec 13.
Laryngeal cancers are the most frequent cancers of the head and neck region. While recent observations indicate the occurrence of an epigenetic field defect in head and neck cancer patients, a detailed exploration of the characteristic changes in the DNA methylation profile in laryngeal cancer patients was lacking. The aim of this study was to assess the methylation frequency of seven genes in a group of patients with primary laryngeal squamous cell carcinoma. Along tumor sections, matching samples of normal mucosa from epiglottis and trachea were analyzed. Gene methylation was assessed using the methylation-specific polymerase chain reaction. We found frequent gene hypermethylation in both the tumor and normal mucosa samples. The methylation of MGMT in tumor cells was associated with lymph node involvement. We report that laryngeal squamous cell carcinomas are characterized by frequent hypermethylation of DAPK, RARbeta, MGMT, RASSF1A and FHIT. Moreover, evidence is shown for the occurrence of a large epigenetically changed field of epithelial cells in laryngeal cancer patients. Our findings indicate the high prevalence of epigenetic abnormalities in laryngeal tumors.
喉癌是头颈部最常见的癌症。虽然最近的观察表明头颈部癌症患者存在表观遗传缺陷，但对于喉癌患者 DNA 甲基化谱的特征性变化仍缺乏详细的研究。本研究旨在评估一组原发性喉鳞状细胞癌患者中七个基因的甲基化频率。对肿瘤切片和取自会厌和气管的正常黏膜的匹配样本进行分析。采用甲基化特异性聚合酶链反应评估基因甲基化。我们发现肿瘤和正常黏膜样本中均存在频繁的基因高甲基化。肿瘤细胞中 MGMT 的甲基化与淋巴结受累有关。我们报告称，喉鳞状细胞癌的特征是 DAPK、RARbeta、MGMT、RASSF1A 和 FHIT 频繁高甲基化。此外，还表明喉癌患者的上皮细胞存在广泛的表观遗传改变。我们的研究结果表明，喉肿瘤中存在高度普遍的表观遗传异常。
