死亡相关蛋白激酶（DAPK）基因启动子甲基化与头颈部鳞状细胞癌的关联：一项荟萃分析。
Association between promoter methylation of DAPK gene and HNSCC: A meta-analysis.
作者信息
Cai Fucheng, Xiao Xiyue, Niu Xun, Zhong Yi
机构信息
Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
出版信息
PLoS One. 2017 Mar 1;12(3):e0173194. doi: 10.1371/journal.pone.0173194. eCollection 2017.
BACKGROUND
The death-associated protein kinase (DAPK) is a tumor suppressor gene, which is a mediator of cell death of INF-γ-induced apoptosis. Aberrant methylation of DAPK promoter has been reported in patients with head and neck squamous cell carcinoma (HNSCC). However, the results of these studies are inconsistent. Hence, the present study aimed to evaluate the association between the promoter methylation of DAPK gene and HNSCC.
METHODS
Relevant studies were systematically searched in PubMed, Web of Science, Ovid, and Embase. The association between DAPK promoter methylation and HNSCC was assessed by odds ratio (ORs) and 95% confidence intervals (CI). To evaluate the potential sources of heterogeneity, we conducted the meta-regression analysis and subgroup analysis.
RESULTS
Eighteen studies were finally included in the meta-analysis. The frequency of DAPK promoter methylation in patients with HNSCC was 4.09-fold higher than the non-cancerous controls (OR = 3.96, 95%CI = 2.26-6.95). A significant association between DAPK promoter methylation and HNSCC was found among the Asian region and the Non-Asia region (Asian region, OR = 4.43, 95% CI = 2.29-8.58; Non-Asia region, OR = 3.39, 95% CI = 1.18-9.78). In the control source, the significant association between DAPK promoter methylation and HNSCC was seen among the autologous group and the heterogeneous group (autologous group, OR = 2.71, 95% CI = 1.49-4.93; heterogeneous group, OR = 9.50, 95% CI = 2.98-30.27). DAPK promoter methylation was significantly correlated with alcohol status (OR = 1.85, 95% CI = 1.07-3.21).
CONCLUSION
The results of this meta-analysis suggested that aberrant methylation of DAPK promoter was associated with HNSCC.
背景
死亡相关蛋白激酶（DAPK）是一种肿瘤抑制基因，是INF-γ诱导凋亡的细胞死亡介质。头颈部鳞状细胞癌（HNSCC）患者中已报道DAPK启动子异常甲基化。然而，这些研究结果并不一致。因此，本研究旨在评估DAPK基因启动子甲基化与HNSCC之间的关联。
方法
在PubMed、Web of Science、Ovid和Embase中系统检索相关研究。通过比值比（OR）和95%置信区间（CI）评估DAPK启动子甲基化与HNSCC之间的关联。为评估潜在的异质性来源，我们进行了meta回归分析和亚组分析。
结果
18项研究最终纳入meta分析。HNSCC患者中DAPK启动子甲基化频率比非癌对照高4.09倍（OR = 3.96，95%CI = 2.26 - 6.95）。在亚洲地区和非亚洲地区中发现DAPK启动子甲基化与HNSCC之间存在显著关联（亚洲地区，OR = 4.43，95%CI = 2.29 - 8.58；非亚洲地区，OR = 3.39，95%CI = 1.18 - 9.78）。在对照来源中，自体组和异质组中均可见DAPK启动子甲基化与HNSCC之间的显著关联（自体组，OR = 2.71，95%CI = 1.49 - 4.93；异质组，OR = 9.50，95%CI = 2.98 - 30.27）。DAPK启动子甲基化与饮酒状况显著相关（OR = 1.85，95%CI = 1.07 - 3.21）。
结论
本meta分析结果表明DAPK启动子异常甲基化与HNSCC相关。
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