Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, China.
Department of Otolaryngology, Ningbo University Medical School, Ningbo, China.
J Clin Lab Anal. 2019 Jul;33(6):e22899. doi: 10.1002/jcla.22899. Epub 2019 Apr 19.
Estrogen-related receptor gamma (ESRRG) has been identified as a tumor suppressor gene in several cancers. We aimed to evaluate ESRRG promoter methylation in laryngeal squamous cell carcinoma (LSCC) and its relative clinical value in LSCC.
Bisulfite pyrosequencing assays were performed on 91 pairs of tumor and paracancer tissues from LSCC patients in China. The diagnostic value and overall survival (OS) were analyzed descriptively by receiver operating characteristic (ROC) curves and the Kaplan-Meier methods, respectively.
The ESRRG promoter was more frequently hypermethylated in tumor tissues than in adjacent tissues (P < 0.01). ESRRG promoter methylation was significantly increased in advanced T stage tumors (P < 0.01) and advanced clinical stage patients (P < 0.01). Moreover, the area under the ROC curve (AUC) value (0.81) indicated high discrimination accuracy. Furthermore, ESRRG hypermethylation was associated with poor OS, as confirmed by Kaplan-Meier survival curves (P < 0.01).
Our study indicated that ESRRG promoter hypermethylation contributed to LSCC-related risks, primarily tumor progression and survival prognosis, in patients. ESRRG promoter methylation could, therefore, be a diagnostic and prognostic biomarker in LSCC.
雌激素相关受体 γ（ESRRG）已被确定为几种癌症中的肿瘤抑制基因。我们旨在评估 ESRRG 启动子甲基化在喉鳞状细胞癌（LSCC）中的作用及其在 LSCC 中的相对临床价值。
在中国 91 对 LSCC 患者的肿瘤和癌旁组织中进行了亚硫酸氢盐焦磷酸测序分析。通过接收者操作特征（ROC）曲线和 Kaplan-Meier 方法分别对诊断价值和总生存期（OS）进行描述性分析。
肿瘤组织中 ESRRG 启动子的超甲基化频率明显高于邻近组织（P<0.01）。ESRRG 启动子甲基化在晚期 T 分期肿瘤（P<0.01）和晚期临床分期患者中显著增加（P<0.01）。此外，ROC 曲线下面积（AUC）值（0.81）表明具有较高的区分准确性。此外，Kaplan-Meier 生存曲线证实 ESRRG 高甲基化与较差的 OS 相关（P<0.01）。
我们的研究表明，ESRRG 启动子甲基化有助于患者的 LSCC 相关风险，主要是肿瘤进展和生存预后。因此，ESRRG 启动子甲基化可以作为 LSCC 的诊断和预后生物标志物。
