曾从事硬煤开采作业工人的肺部病变与血清可溶性 Fas（sCD95）水平

Pulmonary lesions and serum levels of soluble Fas (sCD95) in former hard coal miners.

机构信息

Institute of the Ruhr-University, Bochum, Germany.

出版信息

Eur J Med Res. 2010 Nov 4;15 Suppl 2(Suppl 2):60-3. doi: 10.1186/2047-783x-15-s2-60.

DOI:10.1186/2047-783x-15-s2-60

PMID:21147622

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4360337/

Abstract

OBJECTIVE

Fas/APO-1 (CD95) and Fas Ligand (FasL) is a major mediator system that activates programmed cell death (apoptosis) and is most important for pulmonary cellular homeostasis. Another form of Fas, circulating soluble Fas (sCD95), produced by alternative mRNA splicing antagonizes the cell-surface Fas function. It was the aim of the study to test the hypothesis that the Fas/FasL system is implicated in the development of silica-induced pulmonary nodular lesions.

MATERIAL AND METHODS

We investigated the serum levels of sCD95 in 55 former hard coal miners. Coal workers' pneumoconiosis (CWP) was assumed when the profusion of small round opacities according to the ILO 2000 classification system was 1/1 or greater. Analyses of sCD95 were performed by a sandwich ELISA.

RESULTS

Radiologic CWP was found in 34 of the 55 individuals. The age of subjects with and without CWP was similar (73.5 (SD 7.2) years vs. 73.5 (7.1) years; P = 0.924). sCD95 could be quantified in all samples; significantly higher levels were observed in subjects with radiologic signs of CWP (914 (752-1251) pg/ml vs. 632 (509-804) pg/ml, P < 0.001). However, there was no relationship between sCD95 serum concentrations and the quantity of profusion according to ILO.

CONCLUSIONS

The hypothesis of elevated sCD95 concentrations in CWP was corroborated. The usefulness of sCD95 for prevention and diagnosis of CWP and other forms of silica-induced fibrosis needs to be established by epidemiological studies.

摘要

目的

Fas/APO-1（CD95）和 Fas 配体（FasL）是一种主要的中介系统，可激活程序性细胞死亡（细胞凋亡），对肺细胞的动态平衡至关重要。Fas 的另一种形式是循环可溶性 Fas（sCD95），它通过选择性剪接产生，拮抗细胞表面 Fas 的功能。本研究旨在验证 Fas/FasL 系统是否参与了二氧化硅诱导的肺结节病变的假说。

材料和方法

我们检测了 55 名前硬煤矿工的血清可溶性 Fas（sCD95）水平。根据 ILO2000 分类系统，当小圆形不透明度的密集度为 1/1 或更高时，诊断为煤工尘肺（CWP）。sCD95 的分析采用夹心 ELISA 法。

结果

55 名个体中发现有放射学 CWP 的 34 名。有 CWP 和无 CWP 的个体年龄相似（73.5（7.2）岁 vs. 73.5（7.1）岁；P=0.924）。所有样本均可定量 sCD95；有放射学 CWP 迹象的个体 sCD95 水平显著升高（914（752-1251）pg/ml vs. 632（509-804）pg/ml，P<0.001）。然而，sCD95 血清浓度与 ILO 不透明度的数量之间没有关系。